摘要
本文对引自日本的盘鲍 (H aliotisdiscusdiscus)、皱纹盘鲍 (H.discushannai)和大鲍 (H.gi-gantiea) 3个自然群体的线粒体 DNA 1 6S r RNA基因片段进行了扩增和测序 ,分析了 52 8bp的碱基序列 ,结果显示 :3种鲍的基因序列中 A+T含量为 56.74%～ 57.1 2 %。 3个自然群体间碱基片段序列差异不显著 ,盘鲍与皱纹盘鲍、大鲍彼此均仅有 1处核苷酸检测到变异 ,皆为碱基转换 ,同源性为99.81 % ;皱纹盘鲍与大鲍有 2处碱基转换 ,同源性为 99.61 %。 1 6Sr RNA基因在鲍属内表现出很高的保守性。
mtDNA 16S rRNA Gene fragments of Haliotis discus discus H. discus hannai and H. gigantiea were amplified via PCR, and the PCR products were purified and sequenced. About 528bp nucleotide sequences were retrieved for each of the three species. The content of A+T was at a high level of 56.74%～57.12%. The three corresponding fragment sequences were aligned and compared with each other. There was little difference existing among the three wild species. The mutual homology between Haliotis discus discus vs H. discus hannai, H. discus discus vs H. gigantiea and H. gigantiea vs H. discus hannai was 99. 81%, 99. 81% and 99. 61% respectively. The result shows that the mtDNA 16S rRNA gene fragment sequences of abalone is highly conserved.
出处
《青岛海洋大学学报（自然科学版）》
CSCD
北大核心
2003年第1期36-40,共5页
Journal of Ocean University of Qingdao
基金
中国海洋大学教育部海水养殖重点实验室开放课题 ( 2 0 0 2 0 9)资助
关键词
盘鲍
皱纹盘鲍
大鲍
16S
RRNA基因
序列
线粒体
Haliotis discus discus
Haliotis discus hannai
Haliotis gigantiea
mitochondrial 16S rRNA gene
sequencing
