摘要
目的:检测降钙素基因相关肽(CGRP)、促肾上腺皮质激素(ACTH)、5-羟色胺(5-HT)在正常人淋巴结中的表达,并探讨它们对免疫系统的影响。方法:采用免疫组织化学SABC染色法,检测35例正常人淋巴结(包括反应性增生淋巴结)中CGRP、ACTH、5-HT的表达。结果:35例正常人淋巴结中, CGRP、ACTH及5-HT表达阳性率依次为:88.5％(31/35)、85.4％(30/35)及94.2％(33/35)。3种标记物在淋巴结中的分布大致相同,主要分布在副皮质区的T细胞、淋巴滤泡生发中心B细胞及巨噬细胞等部位。结论:人外周淋巴结中CGRP、ACTH、5-HT的表达率很高,且其分布与免疫细胞的分布基本一致。
AIM: To detect the expression of calcitonin-gene-related pepide (CGRP), adrnocorticotropin(ACTH), and serotonin (5-HT) in normal human lymph nodes and explore their influences on immune system. METHODS; The expression of CGRP, ACTH and 5-HT in the lymph nodes, including proliferative lymph nodes, were detected by SABC Immuno-histochemical staining. RESULTS: In 35 cases of lymph node specimens, the positive rates of CGRP, ACTH and 5-HT expression were 88.5% (31/35), 85.4% (30/35) and 94. 2% (33/35) respectively. These 3 molecules had a similar distribution in germinal center of lymph nodes, mainly in the cells of paracortical region, the cells in germinal center of lymph follicles and macrophages, etc. CONCLUSION; There are considerably high positive rates of CGRP, ACTH and 5-HT in human peripheral lymph nodes, and their distribution area are basically coincident with that of immunocytes in the lymph nodes.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第1期95-96,共2页
Chinese Journal of Cellular and Molecular Immunology
