摘要
应用高效液相色谱－电化学检测法（ＨＰＬＣ－ＥＣＤ）测定大鼠用绵羊红细胞（ＳＲＢＣ）免疫后２～７天期间，脾脏和胸腺中去甲肾上腺素（ＮＡ）、５－羟色胺（５－ＨＴ）和５－羟吲哚乙酸（５－ＨＩＡＡ）含量的变化。结果表明，脾脏和胸腺中ＮＡ含量在免疫后第４和第５天均显著减少；脾脏中５－ＨＴ水平在第３和第４天明显升高，５－ＨＩＡＡ含量在第５和第６天明显增多；胸腺内５－ＨＴ含量仅在免疫后第６天显著增加，５－ＨＩＡＡ水平没有明显变化。以上结果提示，体液免疫应答可影响淋巴器官内单胺类递质的代谢。结合我们以前的研究结果：ＮＡ可影响脾脏Ｔ细胞的增殖，以及外周５－ＨＴ可抑制体液免疫应答，说明单胺类递质与免疫系统之间存在着相互作用。
In this study,we determined the changes of content of noradrenaline (NA),5-hydroxytryptamine(5-HT) and 5-hydroxyindole acetic acid(5-HIAA)in the spleen and thymus of rats immunized with sheep red blood cells (SRBC) during day 2 to 7post-immunization by the aid of the high-performance liquid chrom atography with electrochemical detection (HPLC-ECD). The NA concentration in the spleen and thym us markedly decreased on day 4 and 5 after SRBC injection com pared with the saline-injected control anim als.The content of 5-HT and its metabolite 5-HIAA in the spleen sign ificantly increased,the changes of 5-HT on day 3 to 4 and the changes of 5-HIAA on day 5 to 6 after immunization. the level of 5-HT was markedly high only on day 6 and the level of 5-HIAA did not sign ificantly change during the an tibody response in the thym us.These results indicate that humoral immune response may influence the metabolism of monoamine neurotransmitter in the lymphoid organs.Our previous studies showed that NA can affect the proliferation ofT cells in the spleen and peripheral 5-HT may modulate the humoral immune response of rats. Synthesizing the data of investigation,we consider that the interaction exists between the peripheral monoaminc neurotransmitter and immune system,whiose meaning is keeping the homeostasis of immune function.
出处
《南通医学院学报》
1995年第2期167-170,共4页
ACTA Academiae Medicinae Nantong
关键词
去甲肾上腺素
羟色胺
单胺类递质
神经免疫调节
Noradrenaline
5-Hydroxytryptamine
monoamine neurotransmitter
humoral immune response
neuroimmunomodulation
