摘要
以山苍子精油(LcEO)、壳聚糖季铵盐(CQAS)作原料,利用溶剂法结合高速剪切法制备微米级山苍子精油-壳聚糖季铵盐分散液(LcEO-CQAS-MD),在单因素试验的基础上运用Box-Behnken响应面对制备工艺进行优化,并考察了产品对黄曲霉菌的抑菌活性及其对花生中黄曲霉毒素的影响。研究结果表明:壳聚糖季铵盐与山苍子精油质量比1∶0.8,剪切转速12500 r/min,时间8 min,温度43℃为最佳制备条件,在此条件下所得分散液平均粒径为(381.7±1.8)nm,与预测值(364.465 nm)接近,说明该模型具有较可靠模拟预测性。LcEO-CQAS-MD的多分散系数为0.491±0.015,Zeta电位为(37.4±2.14)mV,分散液稳定性良好,常温保存30 d内无色变、无团聚、无分层现象,再次测量其粒径为(447.6±2.67)nm。此外,测得LcEO-CQAS-MD对黄曲霉的最小抑菌质量浓度(MIC)为500 mg/L,优于单独使用LcEO或CQAS,及市面上常见的抑菌剂,有效降低花生中98.78%的黄曲霉毒素B1。
Using Litsea cubeba essential oil(LcEO)and chitosan quaternary ammonium salt(CQAS)as raw materials,a micronsize L.cubeba essential oil-chitosan quaternary ammonium salt dispersion(LcEO-CQAS-MD)was prepared by the solvent method combined with a high-speed shearing technique.Single-factor experiments were conducted to investigate the effects of the mass ratio of chitosan quaternary ammonium salt to L.cubeba essential oil,shear speed,shear time,and shear temperature,followed by a three-factor,three-level Box-Behnken response surface analysis.The antifungal activity against Aspergillus flavus and its impact on aflatoxin levels in peanuts were also assessed.The results indicated that the optimal preparation conditions for the L.cubeba essential oil-chitosan quaternary ammonium salt micrometer dispersion were a mass ratio of 1∶0.8,a shear speed of 12500 r/min,a shear time of 8 min,and a shear temperature of 43℃.The average particle size of the resulting dispersion was(381.7±1.8)nm,close to the predicted value of 364.465 nm,the polydispersity index(PDI)was 0.491±0.015 and the zeta potential was(37.4±2.14)mV,confirming the reliability of the predictive model.The dispersion liquid has good stability,showing no color change,aggregation,or phase separation during 30 days of storage at ordinary temperatures.Upon remeasurement,the particle size was found to be(447.6±2.67)nm.Furthermore,the minimum inhibitory concentration against A.flavus was found to be 500 mg/L,which outperformed the individual use of L.cubeba essential oil or chitosan quaternary ammonium salt and common antimicrobial agents on the market.It can effectively reduce 98.78%of aflatoxin B1 in peanuts.
作者
张剑涛
陶冉
汪阳东
蒋剑春
陈益存
ZHANG Jiantao;TAO Ran;WANG Yangdong;JIANG Jianchun;CHEN Yicun(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China;College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China;Institute of Chemical Industry of Forest Products,Chinese Academy of Forestry,Nanjing 210042,China)
出处
《林产化学与工业》
北大核心
2025年第6期107-114,共8页
Chemistry and Industry of Forest Products
基金
南京中医药大学中央级公益性科研院所基本科研业务费专项资金(CAFYBB2022ZA002)。
关键词
壳聚糖季铵盐
山苍子精油
分散液
黄曲霉
响应面法
chitosan quaternary ammonium salt
Litsea cubeba essential oil
dispersion
Aspergillus flavus
response surface methodology
