摘要
为推广光学分合法,一种常用于估计器官组织内细胞总数的光学体视框与分合法相结合的无偏体视学方法,本文举一例介绍了其在神经元总数估计中的应用。获得一侧成年小鼠海马结构齿状回(DG区)的连续冰冻切片,等距随机抽选其中1/5的切片(共抽得14张),用免疫组织化学染色结合甲酚紫复染显示DCX阳性神经元,观测切片上的整个DG区,用光学体视框计数15μm厚度的切片组织里DCX阳性神经元的胞体,同时用指针测微计测量切片组织的实际厚度。结果数到291个神经元(胞体),测得切片平均厚度为24.22μm。因此,根据光学分合法,该侧海马DG区DCX阳性神经元的总数为:291×(5/1)×(24.22/15)=2 439。结合这个实例,本文较详细地讨论了光学分合法的理论基础和实践相关问题。
For promotion of the optical fractionator,an unbiased stereological method of the optical disector combined with the fractionator that is commonly used to estimate the total number of cells in an organ(tissue),the present article exemplified its use in a total number estimation of neurons.Serial frozen sections of the dentate gyrus(DG region)of the hippocampal formation on one side were obtained from an adult mouse,1/5 of the sections were sampled in a systematic random manner,with 14 sections being sampled.The sections were stained using an immunohistochemical method and counterstained with cresyl violet to show DCX-positive neurons.The whole DG regions were observed and the somas(cell bodies)of DCX-positive neurons were counted within 15μm(thickness)of the section tissue with the optical disector,meanwhile the actual thickness of the section tissue was measured with a microcator.As a result,291 neurons(somas)were counted and the average of measured section thicknesses was 24.22μm.Therefore,according to the optical fractionator,the total number of DCX-positive neurons in the hippocampal DG region was:291×(5/1)×(24.22/15)=2,439.With this example,we discussed in more details about the theoretical foundation and practice-related issues of the optical fractionator.
作者
何琦
唐勇
杨正伟
HE Qi;TANG Yong;YANG Zheng-wei(Department of Human Anatomy,Research Center of Basic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan;Department of Histology and Embryology,Chongqing Medical University,Chongqing 400016;Morphometric Research Laboratory,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《川北医学院学报》
2025年第8期968-972,共5页
Journal of North Sichuan Medical College
关键词
光学分合法
海马
神经元
数量
体视学
Optical fractionator
Hippocampus
Neurons
Number
Stereology
