摘要
器官内组织结构的量(体积、表面积、长度、数量等)可用体视学(形态定量)方法通过测量组织切片进行估计,但切片组织通常经过了固定、脱水、包埋、切片和染色等处理,测量结果反映的是结构处理后的定量特征。组织处理后结构的体积等可能会发生改变,从而影响体视学估计结果;为了校正结果以反映结构处理前的定量特征,需要分别获得器官组织处理前和处理后的体积以估计组织处理后总的体积变化以及组织内所测结构的相应变化。本文较系统、全面地论述了用处理前后器官体积校正体视学估计的理论基础,如何获得这些体积,如何运用它们校正不同的体视学结果,什么情况下需要单独的校正实验,以及如何进行校正实验。本文的一个重要且新颖之处是引入了一个新概念:组织处理后的器官体积应该分为实际体积和虚拟体积两种,基于三维空间内(实际切片组织内)的测量和基于二维平面上(切片平面上)的测量的体视学估计,要分别基于实际体积和虚拟体积进行校正。
The amounts(such as volume,surface area,length and number)of histological structures can be estimated with stereological(morphometric)methods by measuring tissue sections,but the section tissue has usually undergone processing such as fixation,dehydration,embedding,sectioning and staining and the measured results reflect the quantitative characteristics of structures after the processing.The volume and others of structures will likely change after tissue processing,thus affecting the results of stereological estimation;to correct the results so as to reflect the quantitative properties of the structures before processing,the respective volumes of the organ tissue before and after processing need to be obtained to estimate the overall tissue volume change post-processing and the corresponding change of structures of interest in the tissue.The present paper deals,in a systematic and comprehensive way,with the theoretical basis for correction of stereological estimates using the organ volumes before and after processing,how to obtain the volumes,how to use them to correct different stereological results,in which cases separate experiments are needed for correction,and how to implement the experiments for correction.An important and novel part of the present paper is introduction of a new concept:the organ volume after tissue processing should be categorized into two volumes-actual and virtual volumes;corrections for stereological estimates based on measurements in the three-dimensional space(within the actual section tissue)and those on two-dimensional planes(on sectional planes)should be based on the actual and virtual volumes,respectively.
作者
杨正伟
YANG Zheng-wei(Morphometric Research Laboratory,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《川北医学院学报》
2025年第8期955-967,共13页
Journal of North Sichuan Medical College
关键词
组织处理
包埋
切片
组织学
形态定量
体视学
Tissue processing
Embedding
Sections
Histology
Morphometry
Stereology
