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光镜下突触数——阳性突触素颗粒数的体视学估计 被引量：7

Stereological estimation of the number of synapses—synaptophysin positive granules under light microscope
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摘要 本文介绍了光镜下突触数的体视学估计方法。所观察的突触是用免疫组化技术显示的突触素阳性颗粒;体视学基本方法是光学体视框,即在厚切片内连续"断层扫描"观察并计数突触素颗粒,由此计算其数量。 This article described stereological methods for estimating the number of synapses using light microscopy. The observed synapses are synaptophysin -positive granules shown with immunohistochemical techniques; the basic stereological method is the opti- cal disector,by which the synaptophysin granules are serially scanned, observed and counted within the thick section, and thus their number is calculated.
作者 彭彬 林菁艳 杨正伟
机构地区 川北医学院细胞化学研究室 川北医学院附属医院麻醉科 川北医学院形态定量研究室
出处 《川北医学院学报》 CAS 2013年第3期211-216,共6页 Journal of North Sichuan Medical College
基金 南充市科技局项目(12A0108) 川北医学院科研发展计划项目(CBY12-A-ZD02)
关键词 突触 突触素 体视学 Synapse Synaptophysin Stereology
分类号 R329-3 [医药卫生—人体解剖和组织胚胎学]
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参考文献17

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二级参考文献99

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川北医学院学报
2013年 第3期

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