摘要
目的 广西壮药玉叶金花主要抗炎镇痛药效物质为环烯醚萜苷类的山栀苷甲酯(shanzhiside methyl ester),本研究优化超声波辅助法提取玉叶金花山栀苷甲酯工艺,并研究其体外抗氧化活性。方法 以玉叶金花山栀苷得率为指标,利用单因素试验和响应面试验探究玉叶金花山栀苷提取的最优工艺,工艺因素包括甲醇浓度、提取时间、提取温度及液料比。以DPPH自由基、ABTS自由基和FRAP(总抗氧化能力)为指标考察玉叶金花山栀苷的体外抗氧化活性。结果 超声波辅助法提取玉叶金花山栀苷最佳工艺为甲醇质量浓度55%、温度52℃、液料比(32∶1)mL/g、提取时间98 min,山栀苷甲酯得率为(9.516±1.660)mg/g,回归模型预测的玉叶金花山栀苷甲酯得率为9.532 mg/g,两者相对误差小于0.2%。在抗氧化测试反应5.5 h后,4μmol/L的山栀苷甲酯对DPPH·和ABTS·的清除率分别为95.18%和98.75%,FRAP总抗氧化能力为1.054 mmol/L Vc当量。结论 响应面法可优化玉叶金花山栀苷甲酯的提取工艺,获得的玉叶金花山栀苷甲酯具有优良的体外抗氧化活性。
Objective To optimized the ultrasonic-assisted extraction process of methyl geniposide from Mussaenda pu-bescens and investigated its antioxidant activity in itr0.Methods The yield of shanzhiside methyl ester from Mussaenda pu-bescens was used as an indicator.The optimal extraction process of shanzhiside methyl ester from Mussaenda pubescens was ex-plored using single factor experiments and response surface methodology.The factors included methanol concentration,extrac-tion time,extraction temperature,and liquid-solid rati0.The in vitro antioxidant activity of Shanzhiside methyl ester from Mus-saenda pubescens was evaluated by the scavenging rate of DPPH free radical,hydroxyl radical,and superoxide anion free radi-cal.Results The optimal process for extracting glycosides from Mussaenda pubescens using ultrasound assisted method is meth-anol with a mass concentration of 55%,53℃ extractiontemperature,32:1liquid to material ratio,and extractiontime of 98 mi-nutes.The yield of methyl ester of shanzhiside was(9.516±1.66)mL/g,and the yield of mountain hawthorn glycoside by re-gression model was 9.532 mg/g,with a relative error of less than 0.2%.After 5.5 hours of antioxidant testing reaction,4μmol/L of geniposide methyl ester showed significant effects on DPPH·and the clearance rates of ABTS·were 95.18%and 98.75%,respectively,and the total antioxidant capacity of FRAP was 1.054 mmol/L Vc equivalent.Conclusion Response sur-face methodology can optimize the extraction process of shanzhiside methyl ester from Mussaenda pubescens,and the obtained Shanzhiside methyl ester exhibits excellent antioxidant activity in vitr0.
作者
叶美麟
陈超杰
叶鸿妮
黄燕
庞冬雪
汪可好
Ye Meilin;Chen Chaojie;Ye Hongni(College of Food and Pharmaceutical Engineering,Wuzhou University,Guangzi Zhuang Autonomous Region,Wuzhou 543002,China)
出处
《华中科技大学学报(医学版)》
北大核心
2025年第4期546-551,共6页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金地区基金资助项目(No.81860736)
大学生创新训练项目(No.S202211354157)
广西高校中青年教师科研基础能力提升项目(No.2022KY0680)。
关键词
玉叶金花
山栀苷甲酯
抗氧化
工艺优化
响应面法
Mussaenda pubescens
shanzhiside methyl ester
antioxidant activity
process optimization
response surface method
