摘要
目的观察光化学法诱导大鼠脑梗死形成过程中核转录因子(Nuc lear factor-kappa B,NF-κB)与凋亡调节因子Bax和Bc l-2表达的变化,并探讨山茱萸环烯醚萜苷(iridoid gly-coside,IG)对这一过程的影响。方法大鼠预先灌胃给药7d后,制作光化学致脑梗死模型,采用免疫组织化学法检测脑组织中NF-κB表达的变化,W estern b lot技术检测凋亡调节因子Bax和Bc l-2表达的变化。结果模型组与对照组相比,脑皮层内NF-κB和Bax蛋白表达增多,Bc l-2蛋白表达减少。与模型组相比,IG能够明显的减少NF-κB和Bax蛋白表达,增高Bc l-2蛋白的表达。结论IG可通过影响脑内NF-κB和凋亡调节基因的表达而发挥对脑梗死的治疗作用。
Aim To observe the change of NF-κB and expression of Bax and Bcl-2 related to apoptosis regulation in the cerebral infarction rat and study the protective effect of iridoid glycoside （IG） extracted from cornus officinalis on focal cerebral ischemia injury and its mechanism of action. Methods Rats were pretreated with drugs by mouth for 7 d and a focal cerebral infarction model of rat was induced by photochemical reaction. Immunohistochemistry and western blot were used to detect the change of expression of NF-κB, Bax and Bcl-2 in the cortex. Results Compared with control group, expression of NF-κB and Bax were increased while expression of Bcl-2 was decreased. Compared with model group, IG （IG 20,60,180 mg · kg^-1） markedly decreased the expression of NF-κB and Bax and increased expression of Bcl-2. Conclusion IG has therapeutical effect on cerebral infarction through regulating the expression of NF-κB and apoptosis related genes
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第1期19-22,共4页
Chinese Pharmacological Bulletin
基金
国家重点基础研究发展计划"973"计划资助项目(No2003CB517104)
北京市科技计划项目(NoD0204003000031)
北京市自然科学基金重大项目(No7050001)
北京市中医药重点学科项目(2005)
关键词
环烯醚萜苷
脑梗死
NF-ΚB
BCL-2
BAX
iridoid glycoside
photochemistry
cerebralinfarction
nuclear factor-kappa B
bcl-2
bax
