摘要
本文旨在优化藜蒿秆多糖的提取工艺,评估其体外抗氧化和降糖活性。以藜蒿秆(ASTS)为原料,通过单因素实验结合Box-Benhnken响应面优化藜蒿秆多糖的热水浸提工艺;并对优化提取后的藜蒿秆粗多糖进行脱蛋白处理获得藜蒿秆精制多糖(ASTS-P);以DPPH自由基清除率、ABTS^(+)自由基清除率和总还原能力为指标测定ASTS-P体外抗氧化活性,以α-葡萄糖苷酶抑制率为指标测定其降糖活性。结果显示,藜蒿秆粗多糖热水浸提的最佳提取条件为:提取温度95℃、提取时间3 h、提取次数3次和料液比1:30 g/mL,经验证在该条件下多糖得率为20.92%±0.63%。体外抗氧化实验发现ASTS-P对DPPH自由基、ABTS^(+)自由基的半数抑制浓度分别为0.447、0.214 mg/mL,当ASTS-P的浓度为4 mg/mL时,其总还原能力为1.02;ASTS-P对α-葡萄糖苷酶的半数抑制浓度为9.027 mg/mL,表明ASTS-P具有较好的体外抗氧化活性和一定的降血糖活性。该研究可为藜蒿秆多糖的开发和利用提供参考。
This paper aimed to optimise the extraction process of polysaccharides from amaranth stalks and evaluate their in vitro antioxidant and hypoglycaemic activities.The hot-water extraction process of polysaccharide from Artemisia selengensis Turcz stem(ASTS)was conducted by combining single factor experiment and Box-Benhnken response surface methodology with ASTS as the raw material.The crude polysaccharide was deproteinized to obtain refined ASTS polysaccharide(ASTS-P).The antioxidant activity of ASTS-P in vitro was measured with the scavenging rate of DPPH and ABTS^(+)free radicals and the total reducing power as the indicators.Its hypoglycemic activity in vitro was determined with its α-glucosidase inhibitory activity as the indicator.The optimum hot-water extraction process of crude polysaccharide from ASTS was found to be:extraction temperature 95℃,extraction time 3 h,extraction cycles 3 and solid-liquid ratio of 1:30 g/mL and the yield of the crude polysaccharide under these conditions was 20.92%±0.63%.The half-clearance rate(IC50)of DPPH and ABTS^(+)free radicals of ASTS-P was 0.447 and 0.214 mg/mL,respectively.The total reducing power of ASTS-P was 1.02 when the concentration of ASTS-P was 4 mg/mL.The IC_(50) of ASTS-P against α-glucosidase was 9.027 mg/mL.These results suggested that ASTS-P had good antioxidant activity and certain hypoglycemic activity in vitro.This work may serve as a reference point for the development and usage of polysaccharide from ASTS.
作者
曹建昌
李若彤
曾芬
邹伟
王舒涵
张露
丁翘
CAO Jianchang;LI Ruotong;ZENG Fen;ZOU Wei;WANG Shuhan;ZHANG Lu;DING Qiao(National R&D Center for Freshwater Fish Processing,College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China;School of Health,Jiangxi Normal University,Nanchang 330022,China;State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330027,China;Jiujiang Kai Rui Ecological Agriculture Development Co.,Ltd.,Jiujiang 332000,China)
出处
《食品工业科技》
北大核心
2025年第17期282-291,共10页
Science and Technology of Food Industry
基金
江西省自然科学基金青年基金项目(20232BAB215063)
国家自然科学基金地区项目(32260596)
江西省自然科学基金面上项目(2024BAB25403)
江西省教育厅科学技术研究项目(GJJ210320)
南昌大学食品科学与资源挖掘全国重点实验室开放基金项目(SKLF-KF-202204)。
关键词
藜蒿秆
多糖
响应面优化
抗氧化
Α-葡萄糖苷酶
Artemisia selengensis Turcz stem
polysaccharide
optimization of response surface
antioxidant
α-glucosidase
