摘要
目的:基于“以方(药)测靶”“靶标关联”“由标知效”“临床验效”和“传承创新”组成的中医方药整体作用靶标研究模式,精准阐释三七水煎液在脑病治疗中的作用,发掘其更多潜在新功效。方法:采用热蛋白质组学技术,对三七水煎液在小鼠脑组织中的整体作用靶点进行高通量筛选;利用IPA数据库对三七水煎液作用靶点和下游目标蛋白进行关联研究;进一步运用Genvestigator平台对靶标关联的疾病进行挖掘和分析。结果:共筛选获得三七水煎液直接作用靶点15个、间接作用靶点17个;通过IPA数据库和Genvestigator平台联合分析其关联疾病发现,其中部分靶点参与了缺血性脑卒中、阿尔茨海默病等疾病的治疗,并能够通过神经再生功效修复神经损伤。结论:从靶标角度阐明了三七水煎液治疗脑病的作用机制,提示其具有促进神经再生的新功效。
Objective:Based on the research model for integrative effect of TCM,which consist of modules including"detecting targets of the drug","targeted-regulated molecule association","obtaining the efficacy with the regulated molecule","clinical validation of efficacy",and"inheritance and innovation of TCM",this study gave precise explanation for the function of the Notoginseng Radix et Rhizoma decoction in brain diseases treatment,,and reveals its potential new efficacy..Methods:Thermal proteome profiling was employed for high-throughput screening of the overall targets of this decoction in the mouse brain tissue.The Ingenuity Pathway Analysis(IPA)database was used for the association analysis of the targets of Notoginseng Radix et Rhizoma decoction and the downstream target proteins.Genvestigator was further used to mine and analyze the target-associated diseases.Results:In this study,15 direct targets and 17 indirect targets of Notoginseng Radix et Rhizoma decoction were screened out.The analyses via IPA and Genvestigator showed that some of these targets were involved in the treatment of ischemic stroke and Alzheimer's disease and they were capable of repairing the nerve damage through the new efficacy of neural regeneration.Conclusion:In this study,we elucidate the mechanism of Notoginseng Radix et Rhizoma decoction in treating encephalopathies from the perspective of target and revealed the potential efficacy of this decoction in repairing nerve damage through nerve regeneration.
作者
陈恒
饶沁玲
黄智兴
张鸽
何蓉格
刘嘉彤
梁馨宁
侯毓扬
孔乔慧
董婧
邓欣祺
王春国
CHEN Heng;RAO Qinling;HUANG Zhixing;ZHANG Ge;HE Rongge;LIU Jiatong;LIANG Xinning;HOU Yuyang;KONG Qiaohui;DONG Jing;DENG Xinqi;WANG Chunguo(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国现代中药》
2025年第7期1207-1216,共10页
Modern Chinese Medicine
基金
国家自然科学基金项目(82374076,81703744)
北京市科学技术协会2023—2025年度青年人才托举工程项目(BYESS2023354)
北京市自然科学基金青年科学基金项目(7244493)
北京中医药大学实验技术(操作规程)标准化研究项目(2023-syjs-01)。
关键词
中药靶标
三七
水煎液
热蛋白质组学
作用机制
脑病
神经再生
Chinese medicine target
Notoginseng Radix et Rhizoma
decoction
thermal proteome profiling
mechanism of action
encephalopathy
nerve regeneration
