摘要
本研究以蝴蝶兰‘满天红’(Dtps.Queen Beer‘Red Sky’)无菌苗叶片及茎基部为试材,探讨了叶片不同切割方式、植物生长调节剂、基本培养基及有机添加物对其不定芽诱导、增殖、分化和组培苗生根壮苗培养的影响。研究结果表明叶片边缘切割处理时,不定芽诱导率最高,达46.67%;叶片诱导不定芽适宜的植物生长调节剂组合为2,4-D 1.0 mg/L+TDZ 1.0 mg/L+6-BA 3.0 mg/L+NAA 0.1 mg/L;茎基部诱导不定芽适宜的植物生长调节剂组合为2,4-D 4.0 mg/L+TDZ 0.2 mg/L+6-BA 4.0 mg/L+NAA 0.1 mg/L;不定芽增殖培养适宜的植物生长调节剂组合为2,4-D 1.0 mg/L+TDZ 0.2 mg/L;不定芽生长适宜的植物生长调节剂组合为6-BA 3.0 mg/L+TDZ 0.2 mg/L+NAA 0.1 mg/L;以1/2MS为基本培养基附加100 g/L土豆泥有利于组培苗生根壮苗培养;将蝴蝶兰‘满天红’组培苗驯化一周后移栽成活率为100%。研究结果显示茎基部不定芽诱导所需时间仅为19.47 d,诱导率高达100%,且不定芽数较多,达8.13个,其繁殖效率远高于叶片诱导不定芽,故茎基部更适合不定芽再生途径培养。
In this study,the leaves and stem bases of Dtps.Queen Beer‘Red Sky'sterile seedlings were used as test materials to explore the effects of different cutting methods of leaves,plant growth regulators,basic media and organic additives on adventitious buds induction,proliferation,differentiation,and rooting and strong seedlings culture.The research results showed that the adventitious buds induction rate was the highest when cutting at the leaf edge,reaching 46.67%;the suitable plant growth regulator combination for adventitious buds induction from leaves culture was 2,4-D 1.0 mg/L+TDZ 1.0 mg/L+6-BA 3.0 mg/L+NAA 0.1 mg/L;the suitable plant growth regulator combination for adventitious buds induction from stem bases culture was 2,4-D 4.0 mg/L+TDZ 0.2 mg/L+6-BA 4.0 mg/L+NAA 0.1 mg/L;the suitable plant growth regulator combination for adventitious buds proliferation culture was 2,4-D 1.0 mg/L+TDZ 0.2 mg/L;the suitable plant growth regulator combination for adventitious buds growth culture was 6-BA 3.0 mg/L+TDZ 0.2 mg/L+NAA 0.1 mg/L;the 1/2MS medium supplemented with 100 g/L mashed potato was beneficial for rooting and strong seedlings culture;after one week of acclimatization of Dtps.Queen Beer'Red Sky'tissue culture seedlings,the survival rate of transplanting was 100%.The results of the study showed that the period of adventitious buds induction from the stems base culture was only 19.47 d,the induction rate was as high as 100%,and the number of adventitious buds was more,reaching 8.13,its reproduction efficiency was much higher than that induced from leaves culture,so the stem bases were more suitable for adventitious buds regeneration pathway cultivation.
作者
毛奎静
蘇俊明
崔永一
Mao Kuijing;Su Junming;Cui Yongyi(College of Horticulture Science,Zhejiang A&F University,Lin'an,311300;Deqing Tiandi Agriculture and Forestry Technology Co.,Ltd.,Huzhou,313200)
出处
《分子植物育种》
北大核心
2025年第14期4711-4718,共8页
Molecular Plant Breeding
基金
浙江省自然科学基金项目(Z15C160008)
浙江省农业新品种重大专项花卉育种专项(2021C02071-5)共同资助。
关键词
蝴蝶兰
组织培养
不定芽诱导
生根壮苗
Phalaenopsis
Tissue culture
Adventitious buds induction
Rooting and strong seedlings
