摘要
高效、稳定的杨树叶片再生体系的建立对杨树遗传转化的成功与否起到关键作用。本研究选用白杨优良品种‘北林雄株1号’杨组培苗叶片为材料,利用组织培养技术探索了外植体类型、不同植物生长调节剂种类、浓度、TDZ处理天数、叶龄、光质等条件对‘北林雄株1号’杨叶片不定芽诱导的影响,并对叶片不定芽再生过程进行显微观察,建立并优化了叶片再生体系。结果表明,叶片外植体材料宜选用3~4周的颜色深绿、充分展开,生长点以下第2~4片幼嫩的生根苗叶片,最佳诱导分化培养基为0.4 mg/L 6-BA+0.05 mg/L NAA+0.01 mg/L TDZ,TDZ处理时间9~15 d,不定芽诱导率平均可达到85%左右,最高达94%。本研究优化了‘北林雄株1号’杨组培体系,对叶片不定芽的再生过程进行显微镜观察,为‘北林雄株1号’杨遗传转化研究提供参考。
The establishment of an efficient and stable regeneration system for poplar leaves plays a crucial role in the success of genetic transformation in poplar trees.In this study,the leaves of tissue-cultured seedlings of the elite poplar variety‘Beilin Xiongzhu 1’were used as explants.Tissue culture techniques were employed to investigate the effects of explant type,types and concentrations of plant growth regulators,TDZ treatment duration,leaf age,light quality,and other factors on the induction of adventitious buds.Microscopic observations were conducted on the regeneration process of adventitious buds,leading to the establishment and optimization of a leaf regeneration system.The results indicated that suitable leaf explants are those that are dark green,fully expanded,and 3~4 weeks old,specifically the 2nd to 4th young leaves below the shoot apex of rooting plantlets.The optimal induction medium consisted of 0.4 mg/L 6-BA+0.05 mg/L NAA+0.01 mg/L TDZ,with a TDZ treatment duration of 9~15 days.Under these conditions,the average adventitious bud induction rate reached approximately 85%,with a maximum of 94%.This study optimizes the tissue culture system for'Beilin Xiongzhu 1'poplar and provides a reference for future research on its genetic transformation.
作者
李佳楠
宋宇英
张军
LI Jianan;SONG Yuying;ZHANG Jun(Forestry College of Hebei Agricultural University,Baoding,Hebei 071003,China)
出处
《陕西农业科学》
2025年第5期7-14,共8页
Shaanxi Journal of Agricultural Sciences
基金
“十四五”国家重点研发计划项目“林木良种高效遗传转化技术研究”(2021YFD2200102)
河北省重点研发计划“杨树良种高效基因编辑育种技术研究”(22326318D)。
关键词
杨树
‘北林雄株1号’杨
再生体系
叶片
Populus tomentosa
‘Beilin Xiongzhu 1’
Regeneration system
Leaf
