摘要
探索优化蝴蝶兰组培快繁技术体系,以蝴蝶兰‘大辣椒’半木质化花梗为试验材料,研究消毒剂、基础培养基、植物生长调节剂配比及浓度对蝴蝶兰不定芽诱导、增殖和生根过程的影响。结果表明:1)先用2%H2O2溶液处理15 min,再用10%NaClO溶液处理10 min,消毒效果良好,花梗污染率低,为26.6%;2)不定芽诱导最适培养基为花宝1号+花宝2号+6-BA 3 mg/L+NAA 0.05 mg/L,出芽率为78.9%;3)不定芽增殖最适培养基为花宝1号+6-BA 5 mg/L+NAA 0.05 mg/L,增殖系数为3.60;4)幼苗生根最适培养基为1/2MS+NAA 0.2mg/L,生根率为73.7%。本研究旨在优化‘大辣椒’品种的组培快繁技术体系,为进一步建立蝴蝶兰再生体系和工厂化生产提供技术支持。
In order to develop and optimize the technique system of tissue culture and rapid propagation of Phalaenopsis aphrodite,semi-lignified peduncles of a P.aphrodite cultivar‘Big Chili’were used as test materials to study the effects of disinfectant,basic medium,plant growth regulator on the induction and proliferation of adventitious buds,proliferation,and rooting process.The results showed that 1)the infection rate of peduncles was low(26.6%)after they were treated by 2%H2O2 for 10 min and 10%NaClO for 15 min;2)The optimal medium for adventitious bud induction was Hyponex No.1+Hyponex No.2+6-BA 3 mg/L+NAA 0.05 mg/L,the germination rate was 78.9%;3)The optimal medium for adventitious bud proliferation was Hyponex No.1+6-BA 5 mg/L+NAA 0.05 mg/L,the multiplication coefficient was 3.60;4)The optimum medium for rooting was 1/2MS+NAA 0.2 mg/L,the rooting rate was 73.7%.The results of this study could be used as the technical support for the rapid propagation of P.aphrodite in large scale.
作者
高壮壮
娄倩
刘雅莉
GAO Zhuang-zhuang;LOU Qian;LIU Ya-li(State Key Laboratory of Crop Stress Biology in Arid Areas,Ministry of Agriculture Key Labooratory of Horticultural Plant Biology and Germplasm Innovation,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《西北林学院学报》
CSCD
北大核心
2020年第4期95-100,211,共7页
Journal of Northwest Forestry University
基金
国家自然科学基金(31471905,31701962)。
关键词
蝴蝶兰
不定芽
增殖
生根
快速繁殖
Phalaenopsis aphrodite
adventitious bud
proliferation
rooting
rapid propagation
