摘要
蝴蝶兰花梗腋芽培养在含VW无机盐 +肌醇 10 0mg·L-1+VB110mg·L-1+VB61mg·L-1+烟酸1mg·L-1+蔗糖 2 0g·L-1+BA 5 0mg·L-1的培养基上 2 2℃培养 ,可分化出花葶。花葶切片在含MS无机盐+肌醇 10 0mg·L-1+VB110mg·L-1+VB61mg·L-1+烟酸 1mg·L-1+蔗糖 15g·L-1+BA 5 0mg·L-1+NAA0 5mg·L-1的培养基上诱导分化不定芽。不定芽在同一培养基上继代增殖 ,在附加香蕉汁 10 0g·L-1的Hy ponex 1号生根培养基上壮苗生根。
Inflorescences were induced by culturing flower-stalk buds of Phalaenopsis on V&W medium containing vitamin component of B 5 medium and 5.0 mg·L -1 benzylaminopurine(BA)under 22℃.Adventitious shoot were induced from the internodal segments of inflorescence by using the MS medium containing vitamin of B 5 medium,5 mg·L -1 BA and 0.5mg·L -1NAA.The low sucrose concentration benefits the shoots differentiation and growth.Shoot were propagated on the same medium and developed into plantlet on Hyponex 1 medium containing 100 g·L -1 banana juice and 2 g·L -1 active charcoal.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第2期242-244,共3页
Acta Horticulturae Sinica
基金
中国科学院院长基金资助项目 (76 7)
广东省科技计划资助项目 (C2 0 30 4)
关键词
蝴蝶兰
花葶
离体培养
不定芽
Phalaenopsis
Inflorescences
Adventitious shoot
In vitro culture
