摘要
以冬樱花半木质化茎段为外植体,建立了冬樱花组培快繁体系。冬樱花茎段适宜的消毒方式为先用75%的酒精消毒30s,再用5%次氯酸钠消毒10 min。适宜的初始培养基为MS+1.0mg/L6-BA+0.1 mg/L IBA;最适的增殖培养基为MS+0.3 mg/L6-BA+0.2 mg/LIBA,增殖系数为4.6;最适的生根培养基为1/2MS+0.5 mg/LNAA,生根率为84%,平均根数为6.24条,采用珍珠岩+草炭土+蛭石(体积比1:3:2)基质移栽成活率92%。
A rapid in vitro propagation system was successfully established for Cerasus cerasoides(D.Don)Sok.using semi-lignified stem segments as explants.The optimal surface sterilization method involved sequential treatment with 75%ethanol for 30 s and 5%sodium hypochlorite solution for 10 min.The initial culture medium was MS basal medium supplemented with 1.0 mg/L 6-benzylaminopurine(6-BA)and 0.1 mg/L indole-3-butyric acid(IBA).The optimal proliferation medium was MS containing 0.3 mg/L 6-BA and 0.2 mg/L IBA,yielding a multiplication coefficient of 4.6.Rooting was optimally induced in half-strength MS medium supplemented with 0.5 mg/Lα-naphthalene acetic acid(NAA),resulting in 84%rooting rate with an average of 6.24 roots per plantlet.The survival rate of transplanting using perlite+peat soil+vermiculite(volume ratio 1:3:2)substrate is 92%.
作者
徐丽
张冰
谭钺
宗晓娟
曾沛源
魏海蓉
XU Li;ZHANG Bing;TAN Yue;ZONG Xiaojuan;ZENG Peiyuan;WEI Hairong(Shandong Institute of Pomology,Tai'an,Shandong 271000,China;Daiyue District Forestry Protection and Development Center,Tai'an City,Tai'an,Shandong 271000,China)
出处
《落叶果树》
2025年第3期13-17,共5页
Deciduous Fruits
基金
山东省重点研发计划资助(2024TZXD035,2024LZGCQY013)
现代农业产业技术体系专项资金资助(CARS-30-1-08)
国家自然科学基金面上项目(No.32172534)。
关键词
冬樱花
增殖培养
生根培养
移栽
Cerasus cerasoides
proliferation culture
rooting cultivation
transplant
