摘要
目的 研究托瑞米芬 (TOR)对人肺腺癌细胞系A5 4 9的毒性作用及其与顺铂 (DDP)联用的协同效应 ,探讨肺癌综合治疗的方向。方法 用MTT显色法检测TOR及与DDP联用后对A5 4 9细胞的毒性作用 ,测定其吸光度 (A)值。用流式细胞仪检测细胞DNA含量 ,Westernblot法检测p2 1蛋白表达。结果 TOR能直接抑制A5 4 9细胞的生长 ,≥ 5 μmol L的TOR可明显增强DDP的细胞毒性作用。TOR可加强DDP对S期、G2 期及M期细胞的作用 ,且DDP +TOR后p2 1蛋白表达增加。结论≥ 5 μmol L的TOR与DDP联用对A5 4 9细胞具有显著的协同抗肿瘤效应。
Objective To study the toxic effect of toremifene(TOR) and its synergistic effect with cisplatin(DDP) on human lung adenocarcinoma cell line A549. Methods The cytotoxic effects of these agents on human lung cancer cell line A549 were monitored by a tetrazolium-based colorimetric assay (MTT assay). The cell cycle and DNA content were detected by flow cytometer technic. p21 expression level was monitored by Western blot. Results Toremifene inhibited the growth of A549 cell, with ≥5 μmol/L significantly enhancing the chemosensitivity of cisplatin. TOR enhanced the antitumor activity of DDP at S, G_2 and M phases of cells. And p21 expression was increased after TOR and DDP had been given. Conclusion Toremifene (≥5 μmol/L ) combined with cisplatin shows significant synergistic anti-tumor effect on A549 cells.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第6期537-539,共3页
Chinese Journal of Oncology
关键词
肺癌
托瑞米芬
药理学
顺铂
肺肿瘤
肿瘤细胞培养
Toremifene/pharmacology
Cisplatin/pharmacology
Lung neoplasms
Tumer cell, cultured
