摘要
目的 探讨紫杉醇体外抑制 K5 6 2细胞株及诱导凋亡的作用。方法 体外培养 K 5 6 2白血病细胞株 ,以不同浓度紫杉醇处理 12～ 72小时后 ,用 MTT法测定细胞生长抑制作用 ,用细胞形态学观察和 DNA琼脂糖凝胶电泳检测细胞凋亡 ,用流式细胞仪 (FCM)检测细胞 DNA含量及细胞周期。结果 K5 6 2细胞经紫杉醇处理后细胞生长受抑 ,对 K5 6 2细胞的半数抑制浓度 IC50 为 0 .84μg/ ml。经药物作用后可见细胞染色质浓聚和凋亡小体形成 ,FCM显示细胞凋亡率明显增加 ,但电泳检测未见 DNA裂解。结论 紫杉醇能抑制 K5 6 2细胞的生长 。
Objective To study the effect of taxol on K562 cell proliferation and apoptosis in vitro. Methods K562 cell lines were treated with taxol at different concentration. Cell proliferation was evaluated by MTT and morphological changes of apoptosis were observed with microscopy. Cell apoptosis was investigated by flow cytometry (FCM) and DNA gel electrophoresis. Results The cell growth was inhibited by taxol. The IC 50 value of taxol was 0.84 μg/ml. The typical condensation and fragmentation were observed by microscopy; Apoptotic rate increased by FCM analysis. Conclusion Taxol can inhibit the proliferation of K562 cells in vitro, apoptosis may be one of its antitumor mechanisms.
出处
《实用肿瘤杂志》
CAS
北大核心
2002年第6期376-378,共3页
Journal of Practical Oncology
关键词
白血病
病理学
脱噬作用
抗肿瘤药
紫杉类
肿瘤细胞培养
药物作用
leukemia/pathology
apoptosis
antineoplastic agents
paclilaxel/phamacology
tumor cells,cultured/drug effects
