摘要
目的:通过研究廿烷五烯酸(EPA)对肺腺癌细胞因子和P53表达的影响,探讨其抗肺癌的机制。方法:在细胞培养、动物实验及临床实验应用基础上,检测细胞周期、细胞因子和P53表达。结果:廿烷五烯酸组(EPA)对肺腺癌细胞有显著的抑制作用。EPA作用的肺腺癌细胞周期的G1和S期细胞百分率明显降低,其效能随着EPA作用时间的延长和剂量增大而加强,EPA可引起细胞凋亡。P53表达较对照组明显升高,从而引起G0/G1期阻滞。TNF-α、IL-6、IL-1的血清水平显著低于对照组。结论:EPA可调节机体炎症反应,不仅能提高机体的免疫功能,单剂量应用还可抑制肿瘤细胞生长和活力,而且表现时间和剂量的依赖性。
Objective To study the influence of Eicosapentaenoic Acid （EPA） on the expression of cell factor and p53 in adenocarcinoma of lung and explore the mechanism of against lung cancer. Method On the basis of cell cultivation, animal trial and clinical test application, cell life cycle, expressin of ceil factor and p53 were detected. Results EPA group obviously depressed the growth of adenocarcinoma of lung cell and worked on ceil life circle of the cells whose percentage in stage G1 and S was obviously decreased. The more dose EPA worked on adenocarcinoma, the better affection of cell apoptosis. P53 expression was obviously higher than that of the control group and this led to the obstruction in stage G0/G1. The serumal levels of TNF-α, IL-6 and IL-1 were obviously lower than that in the control group. Conclusion Receiving EPA treatment can accommodate body inflammatory reaction, and enhance body immunity. Single dose application can suppress tumor ceil growth and vitality, further more, show time and dose dependence.
出处
《吉林医学》
CAS
2006年第11期1310-1313,共4页
Jilin Medical Journal
基金
国家自然科学基金资助(30560152)
关键词
廿烷五稀酸
肺腺癌
细胞因子
P53
Eicosapentaepoic Acid
Adenocarcinoma of lung
Cell factor
P53
