摘要
采用高速逆流色谱法分离纯化刺五加粗品中的刺五加苷E。溶剂系统为氯仿 甲醇 异丙醇 水,体积比为5∶6∶1∶4,上相为固定相,下相为流动相,转速为800r/min,流速为2 0mL/min。所得刺五加苷E经高效液相色谱分析,测定纯度为98%(峰面积百分比)。这为其他苷类等极性物质的分离纯化提供了依据。
Analytical highspeed countercurrent chromatography (HSCCC) was used for the systematic optimization of the twophase solvent system to separate eleutheroside E from crude extract of Radix Acanthopanacis Senticosus. Eleutheroside E was obtained by preparative HSCCC with twophase solvent system composed of chloroformmethanolisopropanolwater (5∶6∶1∶4 in volume ratio). The mobile phase is the lower phase and operated at a flowrate of 2.0 mL/min, while the apparatus rotated at 800 r/min. High performance liquid chromatographic analysis of eleutheroside E revealed that its purity was over 98%. HSCCC is a useful method for the separation of natural products.
出处
《色谱》
CAS
CSCD
北大核心
2002年第6期543-545,共3页
Chinese Journal of Chromatography
关键词
有效成分
刺五加苷E
高速逆流色谱法
分离
制备
刺五加
植物药
high-speed countercurrent chromatography
separation
preparation
eleutheroside E
Radix Acanthopanacis Senticosus
