摘要
以显齿蛇葡萄叶为原料,采用微波破细胞进行预处理,然后,采用热水搅拌的方法提取显齿蛇葡萄中的二氢杨梅素.通过透射电镜对显齿蛇葡萄叶细胞微观构象进行观察,探讨不同微波照射条件对植物叶片微观结构的影响来解析微波强化提取机理.结果表明:在300W和微波预处理时间5min或微波功率达到600W和处理时间为2min时,细胞壁出现褶皱并有部分破裂,之后随着照射功率的加大以及微波预处理时间的延长,细胞壁的破裂情况越来越明显.这就说明了细胞内的极性物质在吸收微波能后温度迅速上升,导致细胞内蒸汽压也随之迅速上升,超过了细胞壁膨胀的能力,最终使得细胞壁破裂,从而使细胞内的有效成分的提取分离变得容易.这就是微波辅助提取植物胞内有效成分的提取效率大为提高的原故.
In this paper, the ultrastructure destruction of Ampelopsis grossedntata cell in the process of microwave-assisted solvent extraction was studied. In a series of conditions of differential microwave irradiation strengths and times through the MARS5, the cell wall of Ampelopsis grossedntata leaves were observed with transmission electron microscope. The results showed that with the increase of microwave output power and irradiation time, increasing disruption of cellular structures were found when irradiation time and microwave irradiation strength reached 5 min and 300 W respectively or microwave irradiation time and irradiation strength reached 2 min and 600 W respectively. This is the cause of enhancement of extraction of dihydromyricetin from Ampelopsis grossedntata by microwave.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第5期1198-1203,共6页
CIESC Journal
基金
广东省自然科学基金项目([2003]0730509)
广东省教育厅自然科学基金重点项目(04Z007).~~
关键词
微波辅助萃取
二氢杨梅素
微波萃取机理
显齿蛇葡萄
microwave-assisted extraction
dihydromyricetin
microwave-assisted extraction mechanism Ampelopsin grossedentata
