摘要
目的:测定刺五加浸膏中紫丁香苷、刺五加苷E和异秦皮啶3种有效成分的含量。方法:采用高效液相色谱法,在C18柱上以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速1.0mL·min-1;柱温30OC;检测波长:220nm。结果:被测的3个成分在各自浓度范围内线性关系良好,平均回收率为97.8%～99.0%,RSD为1.8%～2.6%。结论:所建立的方法简便、准确,可用于刺五加浸膏的质量控制。
Objectives：To establish an HPLC method for determination of 3 bioactive components namely syringin,eleutheroside E,isofraxidin in Ciwujia extractum.Methods：Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.1% phosphoric acid solution at a flow rate of 1.0 mL·min-1,column temperature of 30 ℃,and detection wavelength was set at 220 nm.Results：The three compounds in their respective peak areas had good linearities and the average recovery rates were 97.8%-99.0%,RSD was 1.8%-2.6%.Conclusion：The method is simple and accurate,and can be used for the quality control of Ciwujia extractum.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第6期1145-1147,共3页
Chinese Journal of Pharmaceutical Analysis
基金
<中国药典>2010版一部标准研究课题(TS-08)
