摘要
建立测定白酒中γ-氨基丁酸(γ-aminobutyric acid,GABA)和核苷类物质(腺苷、尿苷)的高效液相色谱分析方法。确定检测γ-氨基丁酸的色谱条件为流动相A∶B(A-乙腈;B-50 mmol/L三水合乙酸钠)=35∶65(体积比),流速为1.0 mL/min,柱温40℃,检测波长为436 nm;确定检测核苷类物质的色谱条件为:流动相10%甲醇,流速为0.8 mL/min,柱温40℃,检测波长为254 nm。对20种白酒分析结果显示,不同香型白酒中功能成分差异明显。GABA在浓香型白酒中较高,其中2号酒样中GABA含量达0.867 mg/L。核苷类物质在芝麻香型和酱香型白酒中要高于浓香型,其中9号达到438.960 mg/L。该方法用于白酒中GABA和核苷类物质的分析具有分离效果佳、精密度高、稳定性高的特点。
The high performance liquid chromatography(HPLC)were established for the determination ofγ-aminobutyric acid(GABA)and nucleosides(adenosine and uridine).The conditions of GABA were as follows:mobile phase were acetonitrile and 50 mmol/L sodium acetate trihydrate(35∶65,volume ratio)at a flow rate of 1.0 mL/min,40℃and a detection wavelength of 436 nm.The mobile phase for the determination of the nucleoside substances was 10%methanol at a flow rate of 0.8 mL/min,and the column temperature was 40℃with the detection wavelength of 254 nm.The analyses result of twenty liquor showed that GABA was higher in strong-flavor liquor and detected up to 0.867 mg/L in the sample of 2.The content of nucleoside substances were higher in sesame-flavor liquor and Maotai-flavor liquor than strong-flavor liquor,and the sample 9 showed the highest content(with 438.960 mg/L).The methods were applied to the analysis of GABA and nucleoside substances in liquor with desirable separation,precision and stability.
作者
郭莹
李景辉
李霄霄
李研科
王国明
尹翠娟
张翠英
GUO Ying;LI Jing-hui;LI Xiao-xiao;LI Yan-ke;WANG Guo-ming;YIN Cui-juan;ZHANG Cui-ying(State Key Laboratory of Food Nutrition and Safety Jointly Established by Provincial and Ministry,Key Laboratory of Fermentation Microbiology of Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,College of Biotechnology of Tianjin University of Science and Technology,Tianjin 300457,China;Chengde Bishu Villas Enterprise Group Co.,Ltd.,Chengde 067500,Hebei,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第1期153-158,共6页
Food Research and Development
基金
国家自然科学基金项目(31471724)
天津市应用基础与前沿技术研究计划重点项目(14JCZDJC32900)。
关键词
高效液相色谱法
中国白酒
Γ-氨基丁酸
核苷类物质
检测
high performance liquid chromatography
Chinese liquor
γ-aminobutyric acid
nucleosides
detection
