摘要
目的 研究转化生长因子α和β1(TGF-α,TGF-β1)m RNA在大肠癌 3组不同组织及直肠癌细胞系中的表达 ,探讨其在大肠癌发生、发展中的作用 .方法 分别取 14例大肠癌患者癌灶、癌旁 2 cm及 10 cm之全层肠壁组织 ,采用Northern- blot方法检测 TGF-α,TGF-β1m RNA表达 .结果 14例 3组不同组织及 HR- 8348细胞系均有 TGF- α,TGF-β1m RNA不同程度表达 ,以癌远侧组织中表达最弱 ,同一病例癌远侧组织的表达在 14例中均呈现减弱的趋势 ;在癌远侧组织 A,B期 3例 (2 ,5 ,6号 )和 C期 2例 (8,9号 )杂交信号明显弱于其他例 C,D期信号 .结论 TGF- α及 TGF- β1m RNA表达与大肠癌 Dukes分期相关 ,有可能作为手术切缘的评估指标 .
AIM To investigate the expression of transforming growth factor alpha and beta1 (TGF α, TGF β1) mRNA in 3 kinds of different specimens with colorectal carcinoma and rectal adenocarcinoma cell line, and their effects on the occurrence and development of colorectal carcinoma. METHODS Whole layer intestine tissues, harvested in the regions of the tumor, the paratumor areas (2 cm away from the tumor)and the distal areas (10 cm away from the tumor) from 14 cases with colorectal carcinoma and rectal adenocarcinoma cell line HR 8348 were used to investigate the expression of TGF α and TGF β1 mRNA by means of Northern blot assay. RESULTS ① TGF α and TGF β1 mRNA were expressed in 3 groups of different specimens from 14 cases with colorectal carcinoma. The expression of mRNAs in the distal area were lowest, and in the same case, mRNAs expression showed the decreasing tendency in the distal area among 14 cases. ② The hybridization signals in 3 cases (No 2, 5, 6) with Dukes grade A and B, and 2 cases (No 8, 9) with Dukes C, were lower than those in other cases in distal area. CONCLUSION TGF α and TGF β1 play key roles in oncogensis of colorectal carcinoma, and their expressions are related with Dukes grade which may serve as an potential index in evaluating the excision area for the tumor.
出处
《第四军医大学学报》
北大核心
2002年第21期1958-1961,共4页
Journal of the Fourth Military Medical University
