摘要
目的考察槐定碱对人结肠癌SW620细胞的增殖影响及探讨其诱导凋亡作用。方法MTT法测定槐定碱对SW620细胞的半数抑制浓度;光学显微镜、荧光显微镜及电镜观察细胞凋亡形态变化;流式细胞仪分析细胞周期变化及细胞凋亡率。结果实验结果显示槐定碱对体外培养的SW620细胞增殖具有明显抑制作用,并可诱导其发生凋亡,其作用呈剂量和时间依赖性,48hIC50=2.8mmol.L-1。凋亡细胞表现为细胞固缩、变圆、核染色质聚集或碎裂、胞质空泡化。DNA ladder结果显示有凋亡引起的"梯状"条带出现。流式细胞仪细胞周期分析结果表明,随着槐定碱作用时间的延长,G0-G1期细胞增多,同时S期细胞比例也相应增高。结论槐定碱对体外培养SW620细胞生长增殖有明显抑制作用,其作用机制与阻滞细胞周期的进程,诱导细胞凋亡相关。
Aim To investigate the growth-inhibition and the mechanism of apoptosis induced by sophoridine （SRI） on SW620 cells. Method MTT assay was used to detect the half-inhibition concentration （ICs0）, and fluorescence microscopy, electron microscopy, DNA fragmentation analysis, flow cytometry （ FCM ） were used to demonstrate the presence of apoptosis. Results SRI inhibited the growth of SW620 cells significantly in a doseand time- dependent manner, and morpho- logical characteristics of apoptosis were observed with condensation of nucleus, bubble of cytoplasm, fragment of nucleus . A DNA ladder pattern of internucleosomal fragmentation was observed. Compared with that of control group, the percentage of the G0/G1 phase and the S phase cells increased after treated by SRI. SW620 cells were induced to undergo apoptosis and underwent Go/ G1 arrest with exposure to SRI shown by FCM. Conclusions Sophoridine could induce the inhibition of cell growth by means of apoptosis in a dose-and time- dependent manner, and the blocking of G0/G1 phase of cells was involved in the mechanism of apoptosis.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第6期782-787,共6页
Chinese Pharmacological Bulletin
基金
广州市科技计划资助项目(No2006Z3-E5051-2)
关键词
槐定碱
结肠癌
细胞凋亡
SW620
sophoridine
adenocarcinomaoptosis
SW620colon
ap-optosis
SW620
