摘要
于1988—1989年,对中国对虾的成熟精子用透射电镜和扫描电镜进行了超微结构的研究,结果表明,中国对虾精子系单一棘突类型,主要由前端棘突、中间帽状体和后主体部构成;精子没有尾部,缺少线粒体,不主动运动。比较了来自雄虾体内和雌虾纳精囊内精子的相异之处,发现:(1)雄虾体内精子较粗短,棘突有螺旋结构,而雌虾纳精囊中的则相反;(2)雄虾体内精子核后细胞质带结构更完整,而雌虾纳精囊中精子核后细胞质带囊泡发达并常与质膜融合发生胞吐现象;(3)雄虾体内精子核膜比雌虾纳精囊精子核膜完整;(4)雌虾纳精囊精子的环状片层结构更发达。
The ultrastructure of the spermatozoa in Chinese shrimp, Penaeus chinensis (Osbeck), was studied by scanning and transmission electronic microscopies. The samples were obtained from the seminal vesicle in male animals and from the seminal receptacle (thelycum) in females reared in the overwintering ponds of a shrimp farm during 1988-1989.The unistellate spermatozoa of Chinese shrimp is of the non-motile type. It consists of three main parts: 1. The spike-anterior in position. 2. The cap-shaped part at the middle containing an anterior granule, a special H-shaped structure and membrane pouches. 3. The main-body part at the rear consisted of nucleus and a cytoplasmic band (CB) with vesicles, endo-plasmic reticulum and annulate lamellae; but no mitochondrion is found in the matured spermatozoa. The cap-shaped part combines with the spike to form the acrosomal part of -he spermatozoa.In comparison with that taken from the thelycum in copulated females, the spermatozoa from the seminal vesicle in males has its own morphological characteristics: 1. Its shape is relatively shorter and thicker, possessing a spiral spike. 2. The structure of the cytoplasmic band is more intact, containing fewer vesicles than that from the females. 3. The annulate lamellae in CB is not so strong as that in spermatozoa from females. 4. The nuclear membrane is more intact than that of spermatozoa from females. All the morphological differences imply that capaci-tation process of spermatozoa may happen during its storage in the thelycum of copulated female.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1991年第5期397-401,T002,共6页
Oceanologia Et Limnologia Sinica
基金
中国科学院海洋研究所实验海洋生物开放实验室赞助项目
