摘要
首次报道了中国对虾精子存在有Ｎａ，Ｋ－ＡＴＰ酶及Ｍｇ－ＡＴＰ酶活力，并对 其生物功能进行了探讨。对来自不同季节的雌虾纳精囊中精子的两种ＡＴＰ酶活力 进行了测定。酶分析的结果为春虾精子的 Ｎａ，Ｋ－ＡＴＰ酶活力为 １．１４５±０．２２９μｍｏｌ Ｐｉ／ｍｇ· ｈ，秋虾则为 １． ０５８±０． ４３１μｍｏｌ Ｐｉ／ｍｇ· ｈ；春虾精子的 Ｍｇ－ＡＴＰ酶活力为 １． ６４７±０． ６９１μｍｏｌ Ｐｉ／ｍｍ· ｈ，秋虾则为 １． ５８４±０． ３１５μｍｏｌ Ｐｉ／ｍｇ· ｈ。因此在表观 上，春虾精子的两种ＡＴＰ酶活力均略高于秋虾，但差异不够显著。
This is a first reported that Na. K-ATPase and Mg-ATPase do exist in the spermatozoa of Penaeus chi nensis. The authors further investigated the changes of these enzymes during sperm maturation and/or capacitation in order to find the biological functions of them. The preliminary results showed that the ATPase activity of sperm collected from spring season (Na, K-ATPase: 1. 145± 0. 229 /μmol Pi/mg· h; Mg-ATPase: 1. 647± 0. 991 μmol Pi/mg· h) were higher than those from autumn (Na, K-ATPase: 1. 058± 0. 431 μmol Pi/mg· h; Mg-ATPase: 1. 584 ± 0. 315 μmol Pi/mg· h), however, the difference was not with statistical significance. We have suggested that the changes of ATPase activity in shrimp sperm during autumn to next spring (represent before and after maturation and/or capacitation, respectively) might throw a light of the role of them in these biological process, because it is known that these enzymes are responsible for the energy support and ion flux transmembrance.
出处
《海洋科学》
CAS
CSCD
北大核心
1995年第2期33-36,共4页
Marine Sciences
基金
中国科学院海洋研究所实验海洋生物学开放实验室!(EMBL)资助课题
瑞士LKB公司提供部分资助。
