摘要
于1989－1994年每年4－5月，在福建省漳浦县海区采集长毛对虾，以雄虾精巢为材料，应用光镜和电镜技术研究长毛对虾的精子发生情况。结果表明，长毛对虾精子的发生可划分为精原细胞、初级精母细胞、次级精母细胞、精子细胞及精子5个阶段。精原细胞染色质呈团块状分布在核内膜，胞质电子密度低，呈嗜酸性，细胞紧密排列在一起；初级精母细胞核糖体密度高，胞质呈现嗜碱性；次级精母细胞经历减数分裂变化；精子细胞变态过程主要表现在顶体的形成，顶体由粗糙型内质网演变形成；成熟精子由圆球状的主体部和钉子状的棘突部组成，核染色质属非浓缩型，呈絮状弥散分布于核内，精子全长约为7μm。
This paper describes the stages and the changes of cytological structures of gonad during the oogenesis in Penaeus penicillatus.Based on the size of the cells, the shape of the nucleoli, the existence of the yolk bodies, the emergence of mucous vesicles and relationship between the oocytes and the follicle cells, oogenesis in Penaeus penicillatus can be divided into three stages: oogonia, provitellogenic oocytes and vitellogenic oocytes stages.Oogonium is about 20μm in diameter. Its nucleus big and round in shape. Nucleoli more loose in texture, one or seveial nucleoli in a nucleus. Its ooplasm is transparent, vacant and shows acidophilia.Provitellogenic oocyte is about 20-40μm in diameter. More nucleoli in its nucleus. Nucleoli closely attached to inner nuclear membrane and arranged in ring. Its ooplasm is dense and shows strongly basophilia. The exudates of the nucleoli appeared in ooplasm near the outer nuclear membrane.Vitellogenic oocyte is about 40-300μm in diameter. Many yolk bodies formed and accumulated in its ooplasm. Mucous vesicles appeared in cortex of ooplasm. The oocyte is surrounded by a layer of follicle cells.The relationship between oogenesis and muturity of sex gland, the cytological changes and their significance in oogenesis of P. penicillatus were also discussed.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1999年第4期368-373,共6页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金!39170407
关键词
长毛对虾
精子发生
精子
顶体
penaeus penicillatus
Spermatogenesis
Sperm
Acrosome
