摘要
运用电镜和细胞化学技术研究了日本沼虾精子的形态结构，结果表明；日本沼虾精子属无鞭毛 精子，为单一棘突型，呈图钉样，由主体部和棘突构成；主体部分为帽状体、精核和细胞质带。帽 状体由约２０根辐射状纤丝组成，纤丝上具横纹，并汇合于帽状体基部，由此向后延伸形成棘突；棘 实基部无顶体和中心粒结构，呈ＰＡＳ阴性、快绿阳性反应，精核为非浓缩型，核膜不完整，核质 疏松，快绿和Ｆｅｕｌｇｅｎ阳性，内有许多内质同样膜囊和 ＰＡＳ阳性小泡，分别构成用核的膜状带和泡状带；泡状带中的小泡是精子通过胞饮精荚基质形成的；细胞质带内无细胞器。
The sperm morphology of the freshwater prawn, Macrobrachium nipponense, was in- vestigated by electron microscopy and cytochemical methods. The samples were obtained from the vas deferens of the males and from the spermatophores attached to the ventral body wall of the naturally mated females during 1992-1993. The unistellate sperm of the prawn belongs to aflagellate sperm and resembles a thumb tack. The sperm consists of a main body and a spike. The main body can be distinctly divided into three structural regions: the cap-shaped region, the cytoplasmic band, and the nu- clear region. The cap-shaped region contains about 20 radial fibrils with cross striations. These fibrils converge at the base of the main body and extend to form the spike. The cyto- plasmic band is devoid of centrioles. The nuclear region can be further divided into a vesicu- lar zone and a membranous zone. Many PAS-positive vesicles, formed by pinocytosis of the spermatophore matrix in the vas deferens, characterize the vesicular zone near the concave side of the main body. The membranous zone is filled with membrane cisternae like endo- plasmic reticulum. The nuclear material is loose and shows positive reaction to Feulgen and Fast green. The nuclear envolope is incomplete and indistinct. The spike of the sperm, showing a negative reaction to PAS, does not contain acrosomal complex.
出处
《动物学报》
SCIE
CAS
CSCD
1996年第4期349-354,共6页
ACTA ZOOLOGICA SINICA
基金
国家教委高校博士点科研基金资助
9326906号。
关键词
日本沼虾
精子
超微结构
生殖系统
雄性
形态
Macrobrachium nipponense, Sperm, Ultrastructure, Cytochemistry.
