摘要
目的 探讨脱落细胞形态学定量分析技术在良、恶性胸腔积液鉴别诊断中的应用价值。方法 32例结核性胸腔积液 (A组 )和 4 0例恶性胸腔积液 (B组 )涂片做 HE染色与核仁组成区嗜银蛋白 (Ag NOR)染色 ,并用图像分析仪定量测定 :1A组间皮细胞和 B组肿瘤细胞形态学参数 (面积、周长、最大长径、最小长径、形状因子及核 /浆比 ) ;2 A、B两组 Ag NOR颗粒总面积 /核、形状因子及异形指数。以上定量结果进行统计学比较分析。结果 上述各参数测得值 B组均高于 A组 ,A、B两组间差别具有统计学意义 (P<0 .0 0 1)。结论 脱落细胞图像分析结合 Ag NOR颗粒测定可为良、恶性胸腔积液细胞学鉴别提供量化依据 ,在其辅助诊断中具有实际应用价值。
Objective To evaluate the applied value of computer aided image analysis technology in distinguishing benign from malignant pleural effusions.Methods Smears from thirty two cases of tuberculated pleural effusions(group A) and forty of malignant pleural effusions (group B) were stained by both hematoxylin eosin and AgNOR,respectively. Meanwhile,MIAS were applied to measure the following parameters: cellular area and perimeter; major and minor diameter; morphologic factor;nucleus palsma ratio;AgNOR granule area; AgNOR morphologic factor and AgNOR special shaped index.The morphometric data were studied statistically by means of discriminant analysis.Results All of the parameters mentioned above of group B were significantly higher than those of group A (P<0.001).Conclusions Image analysis of exfoliated cytology combined with AgNOR staining might provide a quantitative basis in distinguishing benign from malignant pleural effusions.It was practicable for applying the technique to the management of patients with pleural effusions or cytological diagnosis.
出处
《中国误诊学杂志》
CAS
2002年第9期1300-1302,共3页
Chinese Journal of Misdiagnostics
关键词
脱落细胞
形态学
定量分析
胸腔积液
恶性
诊断
细胞学
Pleural effusion,naligant/diagnosis
Cytodiagnosis diagnosis
Lung neoplasms/diagnosis
Adenocarcinoma/diagnosis
