摘要
研究射干不同部位的内生菌和根际菌的多样性,探索两者之间的关系,为筛选出功能性菌株奠定基础。采用组织块法和平板稀释法分别分离射干内生菌和根际菌,并对菌株的形态学特征或理化特性与其分子生物学分析相结合进行菌株的鉴定和多样性分析。从射干根茎及叶片中共分离出99株内生真菌和115株内生细菌,鉴定为18个属24个种。射干根茎中的内生菌菌种数(S)、分离频率(IF)、相对分离频率(RF)均高于叶片,而Shannon-Weiner多样性指数(H')和Pielou均匀度指数(E)则低于叶片。射干根茎和叶片中的内生菌ShannonWeiner多样性指数(H')分别为2.32,2.39,两者Sorenson相似系数(Cs)为0.63。从射干根际土壤中分离出91株根际真菌和27株根际细菌,初步鉴定为12个属20个种。根际细菌的菌种数(S)高于真菌,而多样性指数(H')和均匀度指数(E)均低于真菌。根际菌多样性指数(H')和均匀度指数(E)分别为2.49,0.83。射干内生菌和根际菌存在6个共有属和9个共有种,两者相似系数(Cs)为0.41。两者的真菌优势属均为镰刀菌属Fusarium,但两者的细菌优势属分别为鞘脂单胞菌属Sphingomonas、芽孢杆菌属Bacillus。射干内生菌与根际菌的种类多样且分布均匀,具有一定的种属同源性与特异性。
It is aimed to study the diversity of the endophyte in different parts of B. Chinensis (L.) DC and its rhizobacterial strain, and to explore the relationship between the two populations in order to establish the foundation for screening functional strains. The endophyte and rhizobaeterial strainsfrom B. Chinensis were isolated respectively by tissue isolation method and pour plate method. Identification of strains and analysis of diversity were carried out by molecular biological analysis combined with morphological analysis or physicochemical analysis. The results showed that 99 strains of endophytic fungi and 115 strains of endophytie bacteria were isolated from rhizomes and leaves of B. Chinensis, and these strains were identified as 24 species belonging to 18 genera. The number of species （S）, isolation frequency （IF） and relative frequency （RF） of endophyte from rhizomes were higher than those of leaves while the Shannon-Weiner diversity index （H＇） and Pielou evenness index （E） of endophyte from rhizomes were lower than those of leaves. The Shannon-Weiner diversity index (H′） of endophyte in rhizomes and leaves was 2.32 and 2.39, respectively, and the Sorenson similarity coefficient （Cs） was 0.63 in the two organs of B. Chinensis. A total of 91 strains of rhizosphere fungi and 27 strains of rhizosphere bacteria isolated from rhizosphere soil of B. Chinensis were preliminarily identified as 20 species belonging to 12 genera. The number of species （S） in rhizosphere bacteria was higher than that of rhizosphere fungi ,while the diversity index (H′) and the evenness index （E） were lower than those of rhizosphere fungi. The diversity index （H′） and evenness index （E） of rhizobaeterial strain were 2.49 and 0.83, respectively. The similarity coefficient (Cs) of endophyte and rhizobaeterial strain from B. Chirtensis with 9 identical species classified as 6 genera was 0.41. Fusarium was the prevailing fungal genera of endophyte and rhizobaeterial strain while the dominant genera of bacteria were Sphingomonas and Bacillus, respectively. The species of endophyte and rhizobacterial strain from B. Chinensis were evenly distributed with diversity, and it had certain species homology and specificity.
作者
孙未未
王慧
朱艳
秦民坚
SUN Wei-wei;WANG Hui;ZHU Yan;QIN Min-jian(Department of Resources Science of Traditional Chinese Medicines, China Pharmaceutical University, Nanfing 210009, China;State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China)
出处
《药物生物技术》
CAS
2018年第2期123-129,共7页
Pharmaceutical Biotechnology
基金
国家自然科学基金项目(No.81373918)
国家"十二五"规划科技重大专项创新药物研究(No.2012ZX09304006)
中央高校科研业务费项目(No.JKQ2011038)
关键词
射干
内生菌
根际菌
分离
鉴定
多样性
Belamcanda Chinensis（L.)DC
Endophyte
Rhizobacterial strain
Isolation
Identification
Diversity
