摘要
为了丰富射干内生真菌资源,探讨射干内生真菌的生物学特性,本研究从射干Belamcanda chinensis根中分离获得1株内生真菌SG4,通过PCR扩增该菌的ITS区、LSU区基因并进行序列分析。通过平板对峙法测定其对小麦赤霉病菌Fusarium graminearum的抗菌活性,采用十字法测定菌株的最适培养基、最适碳源、最适pH及不同浓度NaCl及重铬酸钾对菌株生长的影响。经鉴定,SG4菌株为尖镰孢菌F.oxysporum,该菌株对小麦赤霉病菌的生长有抑制作用。SG4菌株的最适培养基是Martin培养基,最适碳源是蔗糖,最适生长pH是6~8,对NaCl和重铬酸钾具有一定的耐受性。
To enrich the endophytic fungi resources of Belamcanda chinensis and explore its biological characteristic,an endophytic fungus SG4 is isolated from the root of B.chinensis and its ITS region and LSU gene are amplified by PCR.Its antimicrobial activity on wheat scab(Fusarium graminearum)is determined by plate confrontation method and the effects of the optimal culture medium,the optimal carbon source,the optimal pH,different NaCl and potassium dichromate on the growth of the strain are measured by cross method.SG4 strain is identified as F.oxysporum that has inhibitory effect on the growth of F.graminearum.It has also shown certain tolerance to NaCl and potassium dichromate with Martin medium as the best solid medium,sucrose as the best source of carbon and nitrogen,and 6~8 as the best PH range.
作者
余周源
罗琴
赵欢
杨在君
刘水蓉
彭正松
YU Zhouyan;LUO Qin;ZHAO Huan;YANG Zaijun;LIU Shuirong;PENG Zhengsong(College of Life Sciences,China West Normal University,Nanchong Sichuan 637009,China;School of Agricultural Science,Xichang College,Xichang Sichuan 615000,China)
出处
《西华师范大学学报（自然科学版）》
2020年第1期30-34,共5页
Journal of China West Normal University(Natural Sciences)
基金
四川省教育厅重大培育项目(15CZ0015)
西华师范大学博士启动基金(15E025)
西华师范大学英才科研基金项目(17YC364)。
关键词
射干
尖镰孢菌
分子鉴定
生物学特性
Belamcanda chinensis
Fusarium oxysporum
molecular identification
biological characteristics
