摘要
目的 :探讨睡眠剥夺对大鼠学习能力的影响。方法 :采用小平台水环境法建立睡眠剥夺模型 ,以正常组和大平台组作为对照 ,检测大鼠海马一氧化氮 (NO)及一氧化氮合酶 (NOS)含量变化 ,并以Y型迷宫测量大鼠学习能力。结果 :(1)睡眠剥夺 1d、3d、5d组海马NO含量分别为(5 .2 6± 1.34)、(7.6 3± 1.86 )、(7.96± 2 .15 ) μmol·mgprot- 1,NOS含量分别为 (2 .4 6± 0 .6 8)、(2 .97± 0 .73)、(3.18± 0 .82 )U·mgprot- 1,分别高于正常对照组〔(3.4 9± 1.18) μmol·mgprot- 1、(1.32±0 .6 7)U·mgprot- 1〕和大平台组〔(4.35± 1.0 9) μmol·mgprot- 1、(1.89± 0 .6 2 )U·mgprot- 1(P <0 .0 1,0 .0 5 )〕 ;后两组比较无显著性差异。 (2 )睡眠剥夺 3d、5d组迷宫正确反应所需电击次数分别为 (2 8.1± 5 .2 )、(41.4± 7.9)次 ,所需时间为 (136 .4± 2 1.7)、(186 .7± 2 9.8)s ,高于正常对照组的 (2 2 .9± 4 .7)次 ,(10 9.6± 16 .5 )s和大平台组的 (16 .8± 3.5 )次 ,(82 .9± 13.2 )s(P <0 .0 1)。 (3)NO含量与迷宫正确反应所需电击次数及时间有显著正相关关系 (P <0 .0 1)。结论 :睡眠剥夺导致的大鼠学习能力下降可能与NO/NOS的神经毒性作用有关。
Objective:To explore the effects of sleep deprivation on learning ability in rats. Methods:Sleep deprivation was induced in male SD rats by the 'Flower Pot' technique. Controls were housed either in normal cages (CC) or in tanks with round platforms(TC). Learning ability were tested by Y-maze. The levels of nitric oxide (NO) and nitric oxide synthase(NOS) in hippocampus were measured.Results: (1)NO contents in hippocampus in sleep deprivation 1d,3d,5d groups 〔 (5.26±1.34),(7.63±1.86) and (7.96±2.15) μmol·mg prot -1 respectively〕 were significantly higher than (3.49±1.18),(4.35±1.09) μmol·mg prot -1 in normal and platform controls respectively (P<0.05,P<0.01). NOS contents 〔(2.46±0.68),(2.97±0.73) and (3.18±0.82) U·mg prot -1 respectively〕were significantly higher than (1.32±0.67), (1.89±0.62) U·mg prot -1 in both controls (P<0.05, P<0.01). There was no difference of NO/NOS between both control groups . (2) The times of electric shock to get normal response in sleep deprivation 3d,5d groups (28.1±5.2, 41.4±7.9 respectively) were significantly higher than 22.9±4.7, 16.8±3.5 respectively in normal and platform control groups. The learning time 〔 (136.4±21.7), (186.7±29.8)s respectively〕were significantly longer than (109.6±16.5), (82.9±13.2)s in both control groups. (3) There were significantly positive correlation between NO levels and the times of electric shock or learning time. Conclusion: Sleep deprivation-induced damage of learning ability may be related to the neurotoxic effect of NO and NOS.
出处
《解放军预防医学杂志》
CAS
北大核心
2002年第4期241-243,共3页
Journal of Preventive Medicine of Chinese People's Liberation Army
基金
全军医学科研"十五"规划项目 (No .10MA0 19)
