摘要
目的 探讨一氧化氮 (NO)和生长抑素 (SS)在学习记忆过程中的作用及相互作用。方法 大鼠海马微量注射一氧化氮合酶 (NOS)抑制剂 N-ω-硝基 - L -精氨酸 (N -ω- Nitro- L - Arginine,L - NA )后采用 Y型电迷宫观察大鼠学习记忆能力的改变 ,以硝酸还原酶法测定海马 NO含量 ,以放射免疫法测定海马 SS含量 ,并进行学习记忆能力、NO含量和 SS含量相关性分析。结果 海马微量注射 L - NA组大鼠与生理盐水对照组和正常对照组比较 ,海马中 NO、SS含量明显下降 (P<0 .0 1) ,Y型电迷宫测试次数明显增加 (P <0 .0 1) ,L - NA组大鼠海马 NO水平和SS水平呈显著正相关 ,NO、SS水平均与 Y型电迷宫测试次数呈显著负相关。结论 海马中 NO、SS水平的正常是机体实现正常学习和记忆过程的重要因素 ;NO可能通过调节 SS的合成和释放 。
Objective To study the action and interaction of Nitric Oxide(NO) and somatostatin(SS) in the process of learning and memory. Methods Nitric acid redactase and radioimmunity method are taken to detect the content of NO and SS in rat hippocampus after N ω nitro L arginine (L NA),a nitric oxide synthase (NOS) inhibitor,microinjected into hippocampus,and Y maze is used to observe the changes of capability of learning and memory,and a correlative analysis is made. Results NO and SS of hippocampus decreased(P<0.01) and Y maze test times increased(P<0.01) significantly after the microinjection of L NA. The level of NO was positively correlated with that of SS,and the content of NO and SS were negtively correlated with test times of learning and memory respectively. Conclusion A normal level of NO and SS in hippocampus is foundermental for normal function of learning and memory. NO regulates the level of SS to promote the process of learning and memory.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2001年第3期149-151,共3页
Journal of Apoplexy and Nervous Diseases
基金
山东省卫生厅资助课题 ( 1999CA1CJA2 )
