摘要
目的观察锌对小鼠睡眠剥夺(sleep deprivation SD)后休息不同时间大脑皮层一氧化氮合酶(Nitric oxide synthase NOS)的影响。方法用小平台水环境法建立小鼠SD模型,用NADPH-d组化法显示NOS阳性结构的存在。并计数大脑皮层的全部阳性神经元。结果SD后休息0 h组(R 0 h组)、4h组(R4 h组)与对照组(CC组)相比,NOS阳性神经元数显著升高(P<0.01),SD后休息8 h组(R 8 h组)显著下降(P<0.01),其它组差异无统计学意义。SD后休息0h补锌组(R 0 h+Zn组)较R 0 h组、SD后休息4 h补锌组(R 4 h+Zn组)较R 4 h组NOS阳性神经元数显著降低(P<0.01),SD后休息8 h补锌组(R 8 h+Zn组)较R8h组NOS阳性神经元数显著升高(P<0.01),SD后休息12 h补锌组(R 12 h+Zn组)与R 12 h组相比NOS阳性神经元数升高不明显。结论锌可对抗SD引起的NOS活力改变,促进学习记忆的恢复。
Objective To study the effect of Zn on nitric oxide synthase（NOS）in cerebral cortex during different rest time after sleep deprivation（SD）. Methods SD model of the rat was built by the mini-platform water environment. The positive neurons of NOS in cerebral cortex of rats were stained by NADPH-d histochemistry, the total numbers of which were counted. Results Compared with the control group, numbers of the positive neurons of NOS in R0h group and R4h group increased significantly（P〈0.01）whereas decreased in R8h group significantly（P〈0. 01）, and there were no significant differences among the different rest groups. Numbers of the positive neurons of NOS in R0h＋Zn and R4h＋Zn groups decreased significantly compared with that of R0h and R4h groups respectively（P〈0.01）. Numbers of the positive neurons of NOS in R8h ＋ Zn group increased significantly compared with that in R8h group （P〈0. 01）. Numbers of the positive neurons of NOS in R12h ＋Zn group did not differ significantly from that of R12h group. Conclusions Zn may resist changes of NOS activities after SD, suggesting it may improve the recovery of learning and memory.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2007年第6期346-349,共4页
Industrial Health and Occupational Diseases
