摘要
本试验旨在研究太子参茎叶多糖(RPSLP)对环磷酰胺(CY)所致免疫抑制小鼠免疫功能的影响。将100只小鼠随机分组,腹腔注射CY复制免疫抑制小鼠模型,模型小鼠经RPSLP灌胃后,制备脾淋巴细胞悬液,测定脾淋巴细胞的增殖;收集血清,检测相关免疫指标。结果显示,与CY模型组相比,RPSLP小鼠脾脏指数极显著降低(P<0.01),胸腺指数、吞噬指数α、脾细胞增殖指数SI、免疫球蛋白(IgA、IgG、IgM)、补体(C3、C4)和细胞因子(IL-2、IL-4、IL-6、IFN-γ)含量均不同程度升高(P<0.05或P<0.01)。以上结果提示RPSLP能够提高CY所致免疫抑制小鼠的免疫功能。
This study was designed to elucidate the immuno-enhancement effects of Radix pseudostellariae stem and leaf polysaccharide （RPSLP） in immunosuppressed mice induced by cyelophosphamide （CY） treatment. 100 mice were ran- domly divided, the immunosuppressed mice were given CY via intraperitoneal injection. Then the ratio of splenoeytespro- liferation in immunosuppressed mice was assayed and the immunological indexes in serum of mice were detected. The re- sults showed that compared with CY model group, the spleen index in RPSLP group of mice was significantly decreased （ P 〈 0. O1 ） ; the thymus index, phagocytic index, splenocyte proliferation, immunoglobulin （ IgA, IgG, IgM ）, complement （ Cs, C4 ） and cytokines （ IL-2, IL-4, IL-6, IFN-~） content were significantly increased in different degree （ P 〈 0.05 or P 〈 0. O1 ）. In conclusion, RPSLP enhanced the immune function of immunosuppressed mice induced by CY treatment.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第12期2134-2140,共7页
Natural Product Research and Development
基金
福建省科技厅产学研专项(2014N5004)
关键词
太子参茎叶
多糖
环磷酰胺
免疫抑制
Radix pseudostellariae stem and leaf
polysaccharides
cyclophosphamide
immunosuppression
