摘要
目的 :验证空间学习记忆是否能引起海马苔藓纤维抽芽。方法 :用Morris水迷宫及Timm显影染色方法 ,研究正常成年Wistar大鼠进行定位航行试验后海马CA3区苔藓纤维分布的可塑性变化。结果 :大鼠经 1 4天学习训练 ,其逃避潜伏期逐日缩短 ,由 39 8± 1 5 2s下降到 1 0 9± 7 6s。苔藓纤维主要集中在门区和CA3透明层 ,但在学习组 1 0只和对照组 1 0只大鼠 ,均发现在CA3区始层多少不等地存在苔藓纤维终扣分布 ,两组苔藓纤维分布面密度分别为平均 0 0 5 89± 0 0 335 9,0 0 71 7± 0 0 5 2 84。经“t”检验 ,P >0 0 5 ,差异无显著性。结论 :成年Wistar大鼠Morris水迷宫 1 4天空间学习记忆未见引起海马CA3区苔藓纤维抽芽。
Objective:To test if mossy fibre sprouting can be induced by spatial learning and memory in adult Wistar rats. Methods: Morris water maze was used to train rats by spatial navigation test,and Timm stain was employed to reveal the mossy fibre in hippocampus. Results:During 14 d training,the latency time shortened gradually,from (39.8±15.2)s to (10.9±7.6) s. Mossy fibre was mainly in the hilus and stratum lucidum of CA3. But in stratum orien of CA3 some teminals of mossy fibre can also be found in the learning and the control groups, the numerical density of area were 0.0 589±0.03 359,0.0 717±0.05 284 respectively.The difference between the 2 groups was not significant( P >0.05) by 't''teet'.Conclusion:Fourteen d spatial learning and memory of adult Wistar rats in Morris water maze can not induce mossy fibre sprouting.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2002年第4期379-383,共5页
Chinese Journal of Anatomy
关键词
海马
苔藓纤维
学习记忆
大鼠
hippocampus
mossy fibre
learning and memory
rat
