摘要
目的:建立桑椹颗粒中绿原酸的含量测定方法。方法:采用薄层鉴别法(TLC)对制剂中绿原酸进行定性鉴别;以HPLC法测定桑椹颗粒中绿原酸的含量,采用Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm);使用乙腈-3m L·L^(-1)磷酸水溶液(15∶85)为流动相进行梯度洗脱;流速为0.8 m L·min^(-1);以327 nm为检测波长;柱温为室温。结果:绿原酸的薄层鉴别具有良好的专属性;绿原酸的回归方程:Y=42256X-9219.6,r=0.9994,线性范围为1.300~312.000μg·m L^(-1);平均回收率为96.62%,RSD%值为1.05%。结论:本文建立了桑椹颗粒中绿原酸的HPLC含量测定方法,结果准确,重复性好,可作为桑椹颗粒的质量评价方法。
Objective： To establish a method on content determination of chlorogenic acid component in Mulberry Particles by high performance liquid chromatography （HPLC）. Methods： The preparation chlorogenic acid was identified by TLC qualitatively. A high performance liquid chromatography （HPLC） method was used, and Kromasil C,s （250mm×4.6mm,5 μm） column was used, using acetonitrile-3 mL·L-1 phosphoric acid solution （15：85） as mobile phase in a gradient elution mode. Flow rate was 0.8 mL·min-1, detection wavelength was 327 nm, and column temperature was set at indoor temperature. Results： Chlorogenic acid TLC showed good speci- ficity. Chlorogenie acid regression equation was Y=42256X-9219.6 and r=0.9994 in the concentration range of 1.300-312.000 μg·mL-1, and average recovery was 96.62%,and its RSD was 1.05%. Conclusion： The method is accurate, reproducible and suitable to be used for quality assessment of Mulberry Particles.
作者
刘洋
周玉
肖会敏
何悦
赵延波
王四旺
唐志书
LIU Yang ZHOU Yu XIAO Hui-min HE Yue ZHAO Yan-bo WANG Si-wang TANG Zhi-shu(Shaanxi University of Chinese Medicine, Xianyang Shaanxi 712000, China Ankang People＇s hospital, Ankang Shaanxi 725000, China Institute of Materia Mediea, College of Pharmacy, Fourth Military Medical University, Xi＇an Shaanxi 710000, China Shiyan People＇s hospital, Shiyan Hubei 442000, China.)
出处
《中医药导报》
2017年第1期63-65,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
陕西省"13115"科技创新工程工程技术研究中心组建计划项目(2013ZDGC-01)
