摘要
目的:研究莪术油中吉马酮在小鼠各组织中的分布特征,为莪术油的进一步应用提供参考。方法:取30只KM小鼠ig莪术油0.5 m L,分别于给药1、2、4、8、12 h后随机取6只,采用高效液相色谱法测定其心、肝、脾、肺、肾组织中吉马酮含量;取15只KM小鼠,给药法及取样方法同上,每个时间点取3只,采用荧光法观察上述组织切片中吉马酮的荧光强度;两个实验均取相同数量小鼠作为对照。结果:1~4 h各组织吉马酮浓度随时间的延长而逐渐增加,4 h时达到峰值,且肝、脾浓度明显高于心、肺,吉马酮在各组织内的浓度依次为肝>脾>肾>心>肺;各组织荧光强度观察结果与上述结果一致。结论:两种方法考察吉马酮在小鼠组织中的分布特征一致,证明吉马酮在肝、脾、肾中分布较多,在心、肺分布较少。
OBJECTIVE： To study the distribution characteristics of germacrone from zedoary turmeric oil （ZTO） in each tis- sue of mice, and to provide reference for further application of zedoary turmeric oil. METHODS ： 30 KM mice were given zedoary turmeric oil 0.5 mL; 6 mice were randomly selected 1, 2, 4, 8, 12 h after medication, respectively. The contents of germacrone in heart, liver, spleen, lung and kidney tissues were determined by HPLC. 15 KM mice were selected, medication and sampling method were same as above; 3 mice were collected at each time point respectively. The fluorescence intensity of germacrone in above sections were observed by fluorescence. The same number of mice were selected as control in 2 trials. RESULTS： The con- centration of germacrone in each tissue 1-4 h increased gradually as time and reached the peak value at 4 h. The contents of ger- macrone in liver and spleen were significantly higher than in heart and lung. The concentrations of germacrone in each tissue were ranked as liver〉spleen〉kidney〉heart〉lung. The results of fluorescence intensity observation was same as above results. CON- CLUSIONS： Results of 2 methods show same distribution characteristics of germacrone in mice tissues, and indicate that ger- macrone is distributed more in liver, spleen and kidney tissues and less in heart and lung.
作者
张辉靠
王冬东
孙程
吕晓敏
胡荣
ZHANG Huikao WANG Dongdong SUN Cheng LYU Xiaomin HU Rong(Medical College of Yangzhou Univer- sity, Jiangsu Yangzhou 225001, China)
出处
《中国药房》
CAS
北大核心
2017年第4期512-514,共3页
China Pharmacy
关键词
莪术油
组织分布
吉马酮
高效液相色谱法
荧光观察
Zedoary turmeric oil
Tissue distribution
Germacrone
HPLC
Fluorescence observation
