摘要
为了提纯复壮温郁金资源,快速繁殖良种苗木,满足市场供不应求的现状,对温郁金外植体培养及愈伤组织的诱导、继代、分化和再生进行研究。结果表明:莪术外植体在以MS培养基为基本培养基,附加6-BA 3.0 mg/L+IAA 1.0 mg/L适于丛生芽的诱导与增殖;附加2,4-D2.0 mg/L+KT 5.0 mg/L适于愈伤组织的诱导;附加2,4-D 1.0 mg/L+KT 0.5 mg/L适于愈伤组织的继代培养;附加KT 5.0 mg/L+NAA 0.3 mg/L适于愈伤组织的分化,生根的最佳培养基为MS+IAA 1.0 mg/L。因此,可以通过组织培养的方式提纯复壮温郁金资源,进行温郁金的快速繁殖。
To purify and rejuvenate Curcuma aromatica Salisb resource,reproduce Curcuma aromatica Salisb rapidly,and satisfy the demand of market,we studied the induction,secondary culture and differentiation of callus tissue and explant culture of Curcuma aromatica Salisb.With the different part of Curcuma aromatica Salisb,different hormone,and analyse with Spss software.Results showed： MS medium with 3.0 mg/L 6-BA and 1.0 mg/L IAA was suitable for the induction and proliferation of multiple shoots.MS medium with 2.0 mg/L 2,4-D and 5.0 mg/L KT was suitable for the induction of calli.MS medium with 1.0 mg/L 2,4-D and 0.5 mg/L KT was suitable for the induction of calli.MS medium with 5.0 mg/L KT and 0.3 mg/L NAA was suitable for the differentiation of calli.The best suitable medium for take root was MS ＋ IAA 1.0 mg/L.Thus,we can purify and rejuvenate Curcuma aromatica Salisb resource with the tissue culture,reproduce Curcuma aromatica Salisb rapidly.
出处
《北方园艺》
CAS
北大核心
2008年第10期143-146,共4页
Northern Horticulture
基金
浙江省重大科技攻关资助项目(No.2005C13019)
温州市科技计划资助项目(S2005B001)
瑞安市重大科技资助项目(20052005)
关键词
温郁金
丛生芽
组织培养
快速繁殖
Curcuma aromatica Salisb
Multiple shoots
Tissue culture
Rapid propagation
