摘要
利用手性试剂磺化β-环糊精（S-β-CD）,建立了对药物西酞普兰的毛细管电泳（CE）拆分方法。实验考察了背景电解液（BGE）组成及浓度、pH值、手性拆分试剂种类及其浓度对拆分结果的影响。优化后的分离条件为：BGE采用20 mmol/L柠檬酸＋0.04%S-β-CD（pH=5.50）,采用压力进样0.5psi、5s,分离电压为＋20kV,紫外检测波长为205nm。结果表明：CE对西酞普兰的两个对映体的检出限均为90μg/L,迁移时间和峰面积的相对标准偏差（RSD）都低于3.0%（n=5）,在0.05-100mg/L范围内有良好的线性关系,其线性相关系数（R2）都大于0.998。该方法可成功用于商品药物来士普的质量检测,回收率在103%-108%之间。
A capillary electrophoresis method for chiral separation of citalopram was established using sulfated-β-cyclodextrin as the chiral additive.Several factors on the chiral separation,including the composition,concentration and pH of buffer and the type and concentration of chiral selectors have been studied.The optimized condition was obtained at 0.04%（w/w）sulfated-β-cyclodextrin in 20 mmol/L citric acid.Samples were injected into capillary at a migration stress of 0.5psi and injection time of 5seconds.Separation voltage was＋20kV and detection wavelength was 205 nm.The results showed that the detection limits of both enantiomers were 90μg/L.The relative standard deviations of migration time and peak area were less than 3.0%.Calibration curve was linear over the range of 0.05-100mg/L,and the correlation coefficient was larger than 0.998.This method was successfully used to determine Escitalopram medicine precisely,with average recoveries between 103% and 108%.
出处
《分析科学学报》
CAS
CSCD
北大核心
2015年第4期479-483,共5页
Journal of Analytical Science
基金
中央高校基本科研业务费专项资金(No.2012D10514)
关键词
西酞普兰
手性拆分
毛细管电泳
磺化β-环糊精
Citalopram
Chiral separation
Capillary electrophoresis
Sulfated-β-cyclodextrin
