摘要
以糊精作为毛细管电泳手性分离选择剂,对药物西酞普兰对映体的分离进行研究.考察了糊精浓度、缓冲液体系离子强度和pH及分离电压对对映体分离的影响.在糊精7.0%(m/V)、磷酸盐80 mmol/L(pH 5.4)的运行缓冲液中,分离电压20 kV时,西酞普兰对映体分离度达3.9,同时对拆分机理进行了初步探讨.测定S-(+)-西酞普兰原料药中R-(-)异构体的含量,在0.05～4.00 g/L浓度范围内线性关系良好,R-(-)-西酞普兰与S-(+)-西酞普兰的检出限分别为25.3 mg/L和27.3 mg/L,线性相关系数均在0.9970以上;RSD低于3.2%.
Citalopram （CIT） was separated by capillary electrophoresis using dextrin as chiral additive. The effect of the concentration of dextrin, pH, concentration of electrophoretic running buffer and separation voltage were investigated. The optimized conditions were obtained with 20 kV as separation voltage, 7.0% （m/V） dextrin in 80 mmol/L phosphate （pH 5.4 ） as running buffer. Good resolution of citalopram enantiomers was achieved and the Rs was 3.9 under optimal conditions. The mechanism of separation was discussed too. The quantitative analysis of citalopram was investigated. The linear range of concentration of R-（ - ）-CIT was 0. 05 - 4.00 g/L. The limit of detection of two enantiomers was 25.3 mg/L and 27.3 mg/L. The correlation coefficient was more than 0. 9970, and the RSD was no more than 3.2% respectively.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第11期1527-1530,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20375051
20175038)
教育部青年教师奖(教人司[2002]123)资助课题
