摘要
目的观察动脉灌注3-溴丙酮酸(3-Br PA)对兔移植性直肠肿瘤的作用效果。方法将60只移植有直肠肿瘤的新西兰大白兔随机分为低、中、高剂量治疗组及生理盐水对照组,每组各15只。对低、中、高剂量组实验兔分别经导管于肠系膜后动脉灌注0.5 mmol/L、1.0 mmol/L、2.0 mmol/L浓度的3-Br PA各10 ml;对照组灌注等量生理盐水。4 d后活体解剖取出直肠肿瘤,镜下观察肿瘤细胞坏死程度并计算坏死率,评估各浓度3-Br PA对肿瘤的作用效果。结果 60只实验兔完成直肠肿瘤移植、动脉灌注实验,镜下实验兔肿瘤细胞均有不同程度损坏。低剂量组Ⅰ级坏死3只,Ⅱ级坏死11只,Ⅲ级坏死1只,治疗有效率为6.7%;中剂量组Ⅱ级坏死2只,Ⅲ级坏死10只,Ⅳ级坏死3只,治疗有效率为86.6%;高剂量组Ⅲ级坏死2只,Ⅳ级坏死13只,治疗有效率为100%;对照组Ⅰ级坏死15只。中、高剂量组Ⅲ、Ⅳ级肿瘤坏死率、治疗有效率、4级肿瘤坏死水平比较差异有统计学意义(P<0.05),3-Br PA治疗作用明显,而正常肠组织无损伤。结论动脉灌注3-Br PA治疗兔移植性直肠肿瘤有一定疗效,高浓度剂量组肿瘤坏死率和治疗有效率最高,疗效显著。
Objective To investigate the effect of 3- bromopyruvate（3- Br PA） on transplanted rectal tumors in experimental rabbit models. Methods A total of 60 New Zealand white rabbits with transplanted rectal tumor were randomly and equally divided into low- dose（0.5 mmol/L）, medium- dose（1.0 mmol/L）,high- dose（2.0 mmol/L） treatment groups and saline control group with 15 rabbits in each group. Arterial perfusion of 10 ml 3- Br PA with concentration of 0.5 mmol/L, 1.0 mmol/L and 2.0 mmol/L via caudal mesenteric artery was respectively employed for the rabbits of the corresponding treatment group; the control group was perfused with equal amounts of saline. Four days later, rectal tumors were removed by vivisection. The necrosis degree of tumor cells was determined by microscopic examination, and the necrosis rate was calculated. The effect of different 3- Br PA concentrations on the rectal tumor was evaluated. Results The rectal tumor transplantation and transcatheter 3- Br PA or saline perfusion was successfully completed in all 60 experimental rabbits. Microscopically, tumor cells showed different degrees of damage in experimental rabbits.In low- dose（0.5 mmol/L） treatment group, grade Ⅰ necrosis was observed in 3 rabbits, grade Ⅱ in 11 rabbits,and grade Ⅲ in one rabbit; the effective rate was 6.7%. In medium- dose（1.0 mmol/L） treatment group, grade Ⅱnecrosis was seen in 2 rabbits, grade Ⅲ in 10 rabbits, and grade Ⅳ in 3 rabbits; the effective rate was86.6%. In high- dose（2.0 mmol/L） treatment group, grade Ⅲ necrosis was detected in 2 rabbits and grade Ⅳin 13 rabbits; the effective rate was 100.0%. In the saline control group, grade I necrosis was observed in 15 rabbits. Statistically significant differences in tumor necrosis rate and effective rate existed between medium-dose（1.0 mmol/L） treatment group and high- dose（2.0 mmol/L） treatment group（P0.05）. Statistically significant differences in tumor necrosis rate also existed between each other among the four groups with necrosis of grade Ⅰ to grade Ⅳ（P〈0.05）. 3- Br PA had obvious therapeutic effect, while it showed no damage to the normal intestinal tissue. Conclusion For the treatment of transplanted rectal tumor in rabbit models, arterial infusion of 3- Br PA has certain therapeutic effect. In the high- dose group, the necrosis rate and effective rate are the highest, and the therapeutic results are the most significant.
出处
《介入放射学杂志》
CSCD
北大核心
2015年第7期616-620,共5页
Journal of Interventional Radiology
