摘要
目的:通过3-溴丙酮酸作用于HepG-2细胞,观察3-溴丙酮酸对HepG-2细胞增殖和凋亡的影响。方法:MTT法检测细胞增殖,倒置显微镜和透射电镜观察细胞形态,流式细胞术检测细胞周期分布和细胞凋亡。结果:3-溴丙酮酸在25～75μg/mL范围内,对HepG-2细胞的增殖具有明显的抑制作用并呈现剂量依赖性。3-溴丙酮酸处理HepG-2细胞后,倒置显微镜观察到细胞生长稀疏,细胞质透亮度下降,细胞脱落增多;透射电镜观察到染色质固缩、边集,核质内可见空泡。流式细胞术结果显示3-溴丙酮酸可将HepG-2细胞阻滞于S期,且DNA直方图上可见亚二倍体峰。在3.125～25μg/mL范围内,3-溴丙酮酸可剂量依赖性的诱导HepG-2细胞凋亡。结论:3-溴丙酮酸抑制HepG-2增殖并诱导HepG-2凋亡。
Objective：To investigate the effects of 3-bromopyruvate on the proliferation and apoptosis in HepG-2 Cells.Methods：Cell proliferation was assessed by MTT assay.The morphologic changes of HepG-2 cells were observed with convert light microscope and transmission electron microscope.The cell cycle distribution and apoptotic rate of HepG-2 cells were detected with flow cytometry.Results：The proliferation of HepG-2 cells was inhibited by 25~75 μg/mL 3-bromopyruvate in a dose-dependent manner.HepG-2 cells were treated with 3-bromopyruvate for 48h,convert light microscope showed cell growth was sparse,clarity of cytoplasm decreased and dead cells increased.Trasmission electron microscope showed that HepG-2 cell presented some morphologic features of apoptosis,including nuclear condensation and plasma membrane blebbing.HepG-2 Cell cycle progression was blocked by 3-bromopyruvate in the S pahse and apparent apoptotic peaks were observed.The concentoation of 3.125~25 g/ml 3-bromopyruvate can efficiently induce apoptosis of HepG-2 cells in a dose-dependent manner.Conclusion：3-bromopyruvate can efficiently inhibit the proliferation and induce apoptosis in HepG-2 cells.
出处
《广西医科大学学报》
CAS
2010年第2期183-187,共5页
Journal of Guangxi Medical University
基金
辽宁省教育厅高校科研项目计划(2009T003)
