摘要
目的:研究3-溴丙酮酸(3-BrPA)对宫颈癌HeLa细胞生长及增殖的影响。方法:HeLa细胞经不同浓度3-BrPA作用后,用四甲基偶氮唑蓝(MTT)检测HeLa细胞的增殖情况,倒置显微镜及荧光显微镜观察细胞形态,流式细胞仪检测细胞周期分布及坏死或凋亡情况。结果:经3-BrPA作用一段时间后:MTT检测发现在一定浓度范围内(50-200)μmol/L对HeLa细胞增殖的抑制作用呈浓度依赖性,与对照组比较,各浓度组差异均有统计学意义(P<0.01)。倒置显微镜下可见HeLa细胞生长稀疏,细胞透亮度下降,细胞皱缩、破碎。Hoechst染色后荧光显微镜下呈现典型的凋亡核固缩表现。细胞周期分析结果表明,可诱导HeLa细胞G2/M期阻滞(P<0.01)。流式细胞仪及荧光显微镜观察表明3-BrPA可诱导细胞坏死和凋亡(P<0.01)。结论:3-BrPA对宫颈癌细胞增殖有显著抑制作用。
Objective： To observe the inhibitory effect of 3 -bromopyruvate （3 - BrPA） on the cell proliferation of HeLa human cervical cancer cell line. Methods： The HeLa cells were exposed to 3 - bromopyruvate environment with different concentrations and different times, then cell growth was measured by MrIT, the cell cycle distribution and apoptotic or necrotic cell death were detected with flow cytometry. The morphologie changes of HeLa cells were ob- served with convert light microscopy and fluorescent staining. Results：3 -bromopyruvate （ 〉 50p^mo]/L） significantly inhibited the proliferation of HeLa cells in a does -dependent manner, induced GJM phase arrest（P 〈 0.01 ）, cell necrosis and apoptosis （P 〈0.01 ）. The HeLa cells were treated with 3 -bromopyruvate for 24h, convert light mi- croscopy showed cell growth was sparse, clarity cytoplasm decreased and dead cell increased. Conclusion： 3 - bro： mopyruvate can efficiently inhibit the proliferation of Hela cells.
出处
《现代肿瘤医学》
CAS
2012年第5期921-924,共4页
Journal of Modern Oncology
