摘要
目的：为了建立定量检测酪蛋白水解物中的血管紧张素转换酶（ACE）抑制肽的反相高效液相色谱（RP-HPLC）方法。方法：酪蛋白先经过胃蛋白酶水解、再经过胰蛋白酶水解,用风味蛋白酶和离子交换树脂对水解物先后进行脱苦和脱盐处理。建立标准品十肽酪氨酸-谷氨酰胺-赖氨酸-苯丙氨酸-脯氨酸-谷氨酰胺-酪氨酸-亮氨酸-谷氨酰胺-酪氨酸（YQKFPQYLQY）的RP-HPLC检测方法,运用外标法测定酪蛋白水解物中的ACE抑制肽YQKFPQYLQY的含量。结果：标准品十肽YQKFPQYLQY质量浓度在2.5~4.5mg/m L（R2=0.95496）范围内与峰面积呈良好的线性关系。酪蛋白水解物中所含十肽质量分数为4.96694%±0.00131%。结论：本液相检测方法操作简便、快速,适用于ACE抑制肽YQKFPQYLQY的定量检测分析。
The purpose was to establish a method for quantitative determination angiotensin converting enzyme （ACE） inhibitory peptides in hydrolyzate of casein using reversed-phase high performance liquid chromatography （RP-HPLC）. Casein was hydrolyzed by pepsin,and then was hydrolyzed by trypsin. With flavourzyme for debittering of casein hydrolyzate. Salts of casein hydrolyzate were removed by ion exchange resin. The RP- HPLC method to detect decapeptide YQKFPQYLQY was established. Using external standard method to determine YQKFPQYLQY of casein hydrolyzate. Results showed that,the linear range of decapeptide was 2.5~ 4.5mg/mL（FF=0.95496）. The decapeptide concentration in the casein hydrolyzate was 4.96694%_＋0.00131%. Therefore,the RP-HPLC was simple and rapid,suitable for the quantitative detection of ACE inhibitory peptides YQKFPQYLQY.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第10期54-58,共5页
Science and Technology of Food Industry
基金
天津市科技支撑项目(14ZCZDNC00017)
天津市高等学校创新团队项目(TD12-5049)
天津市应用基础与前沿技术研究计划重点项目(14JCZDJC34500)
关键词
酪蛋白
水解物
血管紧张素转换酶抑制肽
定量检测
反相高效液相色谱法(RP-HPLC)
casein
hydrolyzate
angiotensin converting enzyme inhibitory peptide
quantitative determination reversed phase-high performance liquid chromatography（RP-HPLC）
