摘要
本文报告3种化学剂与2种药物在体内与体外杀灭原头节的效果。终浓度为0.3％的北美黄连碱与原头节作用15min后,头节死亡率分别为68.9％和70.2％;终浓度为10％的乙醚-醋酸-乙醇混合液(乙醚:醋酸:乙醇为5:3:2)分别为56.2％和56.8％,较终浓度为0.3％的H_2O_2的杀头节效果(6.0％)为佳,0.004％吡喹酮与0.004％阿苯达唑在体外试验中与原头节作用15min未见有杀灭原头节效果。初步结果表明北美黄连碱和乙醚-醋酸-乙醇混合液具有较迅速和较强杀原头节作用。扫描电镜观察结果,经北美黄连碱作用后,原头节顶突质膜皱襞断裂,头钩排列紊乱、脱落,吸盘变形,头钩与吸盘表面膜内颗粒减少。
The present paper reports the protoscolicidal action of hydrastine, ether-acetic acid-1 ethanol admixture, H2O2, pyquiton and albendazole through in vitro or in vivo exposue, for 15 minutes and transplantation studies. The mortality of protoscblices in vitro and in vivo were 70.2% and 68.9% for 0.3% hydrastine, 56.8% and 56.2% for 10% ether-acetic acid-ethanol admixture, 6.0% and 8.8% for0.3% H2O2; 6.1% for 0.004% pyquiton in vitro and 5.0% were 10% and 25% for 0.3% hydrastine, 30% and for 0.004% albendazole in vitro. The survival rates after transplantation of protoscolices 37.5% for 10% ether-acetic acid-ethanol admixture, 100% and 95% for 0.3% H2O2 respectively. Disruption of external plasma membrane, hook detachment, sucker deformitv of protoscolices exposed to hydrastine were demonstrated by scanning electron microscopy. It is suggested that hydrastine exerts a profound intracellular effect on the protoscolex of E. granulosus of sheep and man, and might be a promising protoscolicide as adjuvant to hydatid surgery.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1991年第2期137-139,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
细粒棘球绦虫
原头节
化学剂
药物
Echinococcus granulosus, protoscolex, hydrastine, ether-acetic acid-ethanol admixture, pyquiton, albendazole, scanning electron microscope.
