摘要
从 Mitchell 实验室引进与我们报告的24—26kD 抗原类似的重组 Sj26抗原基因的 E coil,从中抽提并纯化出 rSj26抗原。重组 Sj26抗原初步应用的结果表明:(1)rSj26抗原 ELISA 可以检测日本血吸虫大陆株感染鼠血清中特异性抗体,最高滴度1:320,不比同种24—26kD 抗原检测的效果差;(2)用24—26kD 抗原检测 rsj26免疫鼠血清中的抗体水平,和以 rSj26抗原检测的水平相似,而用 SEA 测抗 rSj26抗体水平较差}(3)用 rSj26免疫鼠后以大陆株尾蚴攻击感染,减虫率可达44.4%,表明 rSj26可诱导一定程度保护性免疫力预防日本血吸虫大陆株尾蚴感染。对 rSj26抗原引进的重要意义进行了讨论。
We introduced from Dr.Mitchell s laboratory the engineering bacteria of recombinant Sj26 antigen gene which is extremely similar to 24—26 kD antigen purified from Chinese strain of S.japonicum.The ex- traction,purification and preliminary application of rSj26 were carried out.The results showed that:(1) rSj26 antigen can be used in ELISA to detect the specific antibody in sera of mice infected with S.japonicum (Chinese strain),The highest titer was 1:320 which was almost same level comparing with 24—26 kD anti- gen.(2)The level of antibody in sera of mice immunized with rSj26 detected by 24—26 kD antigen was similar to that by rSj26 antigen.The level of antibody against rSj26,however,was lower when using SEA (S.j).(3)The worm reduction rate after challenging infection in mice immunized with rSj26 was as high as 44.4%,and was similar to that in mice immunized with 24—26 kD antigen.It is suggested that rSj26 antigen can induce certain level of specific protective immunity to protect the host against infection by Chi- nese strain of S.japonicum cercariae.The progress in application of rSj26 antigen discussed.
出处
《中国寄生虫病防治杂志》
CSCD
1991年第3期201-204,共4页
Chinese Journal of Parasitic Disease Control
基金
国家科委863高技术计划资助项目
