摘要
为检测血吸虫重组 BCG- Sj2 6 GST疫苗免疫小鼠血清 Ig G动态变化和免疫保护力 ,采用 10 6 CFU疫苗皮下 1次和 3次接种小鼠 ,接种后 8周用日本血吸虫尾蚴攻击感染。感染后 6周剖杀小鼠 ,计算减虫率和减卵率 ,同时设有 BCG对照组。结果发现 :实验组免疫后血清 Ig G抗体迅速升高并持续于高水平 ;减虫率分别为 39.74%和 39.74% ,减卵率分别为 6 2 .86 %和 5 5 .6 2 % ,与对照组相比均有非常显著的差异 (P<0 .0 1)。而免疫 3次和免疫 1次小鼠血清 Ig G抗体水平及减虫率、减卵率均无显著差异。血吸虫重组 BCG- Sj2 6 GST疫苗皮下注射 1次即能诱导小鼠较高水平的 Ig G抗体和免疫保护力 ,是一种比较理想的新型疫苗 。
To study the protection and dynamics of serum IgG in the mice immunized with recombinant BCG Sj26GST vaccine of Schistosoma japonicum , the mice were immunized subcutaneously by 10666 CFU vaccine for one time or three times respectively. Eight weeks later, the mice were challenged with Schistosoma japonicum (Sj) cercariae after the first immunization and killed at the 6th week after challenge. The rate of reduced worm burdens and the rate of reduced eggs number in liver were counted. Besides, the BCG treated mice served as control group. It was found that IgG rose quickly and kept in high level after immunization in the experimental group, with the rate of reduced worm burdens being 39.74 % and 39.74 %, and the rate of reduced eggs number being 62.86 % and 55.62 % respectively. There was very significant difference between the experimental and control groups ( P <0.01). But the level of IgG, the rate of reduced worm burdens and the rate of reduced eggs number had no statistical difference between the mice immunized for one time and those for three times. It was concluded that immunizing the mice once subcutaneously with recombinant BCG Sj26GST vaccine of Sj could induce high level of IgG and protection and was a new type of more ideal vaccine.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第1期4-6,共3页
Acta Universitatis Medicinae Tongji
基金
卫生部总理基金资助重点项目 (No. 94- Y19)
