摘要
采用ＰＣＲ法克隆自身抗原Ｒｏ５２ｋｄ多肽分子的ｃＤＮＡ，定向插入表达载体ＰＧＥＸ－４Ｔ－１，并导入大肠杆菌中表达重组融合蛋白，经免疫印迹法表明，重组融合蛋白具有Ｒｏ５２ｋｄ的抗原性。这为今后对Ｒｏ５２ｋｄ抗原表位的精确定位，分析特定抗原表位与疾病的相关性及制备重组抗原用于临床检测奠定了基础。
y adopting PCR technology, obtained the cDNA clone encoding autoantigen Ro 52 kd. Comfirmed by DNA sequencing, the target cDNA was subcloned unindirectionally into the bacterial expression plasmid PGEX4T1 and then transformed into Ecoli to express the recombinant protein. Further analysis by immunoblotting demonstrated that the recombinant protein exhibited the antigenicity of autoantigen Ro 52 kd.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第1期54-56,共3页
Chinese Journal of Immunology
基金
上海市启明星基金
